Examples of 'blunt ends' in a sentence
Meaning of "blunt ends"
Blunt ends is a term commonly used in biology and genetics to describe the result of a DNA molecule being cut straight across, resulting in flat ends rather than staggered or overlapping ends
How to use "blunt ends" in a sentence
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blunt ends
In some embodiments blunt ends are produced on cDNA.
The enzymes selected produced blunt ends.
Such blunt ends are desirable in some occlusion device embodiments.
Each fragment thus has blunt ends.
Since the inserts contained blunt ends two orientations were obtained of each cloning.
Pupa lack a cremaster and have blunt ends.
On the other hand, blunt ends are always compatible with each other.
In other embodiments siNA molecules have blunt ends.
The PCR product having the phosphorylated blunt ends were recovered as a precipitate.
In the following step PstI linkers are ligated to these blunt ends.
The PCR product having the phosphorylated blunt ends was recovered as a precipitate.
Any residual overhangs were removed by digestion with mung bean nuclease leaving blunt ends.
Screws having eccentric or blunt ends may be used.
After digestion the vector was treated with Klenow enzyme to create blunt ends.
The ends of the siRNA can be either blunt ends or protruding ends.
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Both ends of the fragment were treated with a Klenow DNA polymerase to make blunt ends.
Tagging can comprise attachment to blunt ends or sticky ends.
The fragmented DNA was treated enzymatically to repair and generate phosphorylated and blunt ends.
This problem was circumvented by exploiting the blunt ends typically possessed by PCR products.
This fragment was treated with Klenow enzyme to make blunt ends.
The sticky ends are converted to blunt ends by Klenow DNA polymerase.
The BamHI site was filled in to create blunt ends.
Thus, ligatable sticky or blunt ends should be avoided.
Klenow DNA polymerase was used to make blunt ends.
The religation of the blunt ends causes correct fusion, also verified by sequencing.
Ligation can also be between two blunt ends.
Alternatively, blunt ends in both the insert and the vector may be ligated.
Exonucleases may cleave the phosphodiester bonds at blunt ends or sticky ends.
The blunt ends were ligated and the resulting plasmid designated delta pBR4.
DNA cleavage can result in the production of either blunt ends or staggered ends.
In other words, the blunt ends are the ends of the fully double-stranded section.
DNA cleavage may result in the production of either blunt ends or staggered ends.
Next, the blunt ends are ligated to a kinased Sal I linker.
This plasmid would be cut with Pvu II to generate a linear plasmid with blunt ends.
Providing products having phosphorylated blunt ends derived from said nucleic acid segments of interest ;.
Are ligated to double-stranded cDNAs with blunt ends.
The above two DNA fragments with blunt ends were ligated to construct DON2 vector.
This fragment was treated with Po1Ik to generate blunt ends.
In a preferred embodiment, an increased number of blunt ends are created with Pfu DNA polymerase.
Figure 2 illustrates various combinations of overhangs and blunt ends.
Blunt ends are created by T4 polymerase treatment.
In a first step, products having phosphorylated blunt ends are provided.
Two DNA fragments with blunt ends were ligated to construct DON1.2 vector.
In certain embodiments, the siRNA polynucleotide may have blunt ends.
Linkers may be ligated to blunt ends with T4 DNA ligase.
In one embodiment, double stranded siNA molecules comprise blunt ends.
The double-stranded RNA oligonucleotide has blunt ends or unpaired ends of 2 to 6 nucleotides.
Restrict pBMDL2 with SmaL to linearize it and to obtain blunt ends.
The Asp718 cohesive ends were converted to blunt ends by treatment with mungbean nuclease.
When cleaved by the StuI enzyme, a StuI sequence yields blunt ends.
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Examples of using Blunt
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Blunt trauma to the chest and abdomen
No obvious signs of blunt or sharp force trauma
Blunt force trauma to the temporal bone
Examples of using Ends
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Take the ends of the hair fig
Ends designed to facilitate continuous row mounting
Check that both ends are correctly plugged in
