Examples of 'buffer exchanged' in a sentence
Meaning of "buffer exchanged"
buffer exchanged: This phrase likely refers to a process in which buffers, which are solutions used to maintain a stable pH level, are swapped or replaced. In various scientific or technical contexts, buffer exchange might involve transferring a sample from one buffer solution to another for specific purposes
How to use "buffer exchanged" in a sentence
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buffer exchanged
The antibody samples were buffer exchanged into water.
Following preparation of a cell lysate, said cell lysate may be buffer exchanged.
All of the bulked antibody was buffer exchanged into PBS buffer.
The pooled HIC protein-containing eluate was then concentrated and the elution buffer exchanged.
The column eluate was buffer exchanged into PBS.
Buffer exchanged into deionised water by repeated ultra filtration at 4°C.
The thiolated polysaccharide is buffer exchanged with water.
These samples were buffer exchanged into deionised water by repeated ultra filtration at 4°C.
The biotinylated antibody was then buffer exchanged into PBS.
The purified gp96 was buffer exchanged into PBS and divided in to several aliquots.
Secreted IgG was purified using protein A sepharose ( Pharmacia ) and buffer exchanged into PBS.
Subsequently, each sample was buffer exchanged into saline by tangential flow.
Supernatants were removed, clarified by centrifugation, concentrated, buffer exchanged into PBS.
The ErbB4 ECD was buffer exchanged into PBS and concentrated.
For example, in the follow up experiments the samples were buffer exchanged into Tris-HCl-HCl buffer.
See also
Proteins were buffer exchanged to PBS and concentrated to 1 mg / ml.
Preferably, the IGF / IGFBP is directly buffer exchanged into a pH buffer.
The buffer exchanged antibody was stored for 2 hours at 4 °C.
At this stage, the Con A eluate was buffer exchanged into PBS and divided into 3 samples.
Liposomes were buffer exchanged into HBS, pH 7.4 using a hand-held tangential flow column.
Alternately, the IGF / IGFBP may be directly buffer exchanged into a pH buffer.
The protein fractions were buffer exchanged into 20 mM TEA HCl pH 7.5 using a tangential flow concentrator.
The resulting irinotecan-containing liposomes were buffer exchanged into phosphate-buffered sucrose ( pH 7 ).
The rCS6 was then buffer exchanged with phosphate buffered saline ( PBS ).
Each reaction then had the buffer exchanged for pH 7.4 PBS.
Finally, the eluate was buffer exchanged into PBS pH 7.4 and the concentration determined.
The purified Fab ' was concentrated by ultrafiltration and buffer exchanged into 0.1M potassium acetate pH6.
The material is concentrated and buffer exchanged into formulation buffer using a 10kDa MWCO PES membrane.
This fraction was further purified and buffer exchanged with a Sepharose 4 FF ( Amersham ) column.
Briefly, proteins were buffer exchanged to PBS and concentrated to 1mg/ml.
The anion exchange pooled fractions were buffer exchanged by dilution with PBS/0.02 % sodium azide.
The volume is reduced and buffer exchanged ( PBS ) using a centrifugal concentration device ( Millipore ).
The extract may be optionally buffer exchanged down Sephadex ® G25.
The sample was then buffer exchanged using a PD-10 column ( Amersham Pharmacia ).
Following the HIC step, product was buffer exchanged using tangential flow filtration ( TFF ).
The purified protein was buffer exchanged into PBS using a 30kD MW cut-off concentration unit.
The 354 MAb was then buffer exchanged by diafiltration.
Immunodepleted samples were buffer exchanged using 10 kDa cut-off spin filters.
Eluates were concentrated and buffer exchanged against PBS with centricon 30 microcentrators ( Amicon ).
The liposomes were then buffer exchanged into 0.9 % saline by tangential flow.
The concentrated solution was buffer exchanged into 20 mM sodium citrate buffer at pH 4.3.
The purified protein was buffer exchanged into PBS and filtered using 0.22 µm filter.
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Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
Examples of using Exchanged
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These ones can be exchanged free of charge
We exchanged a scum for a hero of the people
Many of these have been exchanged in a test environment
