Examples of 'cdna insert' in a sentence
Meaning of "cdna insert"
CDNA insert: Refers to a segment of DNA that has been inserted into a vector for cloning or expression purposes. CDNA (complementary DNA) is generated from RNA using the enzyme reverse transcriptase
How to use "cdna insert" in a sentence
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cdna insert
A cDNA insert can be cut out easily.
The thicker line represents the cDNA insert.
The cDNA insert was sequenced on both strands.
The increase in expression depends on the particular cDNA insert.
This cDNA insert was subcloned for sequence analysis.
The positive clone is confirmed by sequencing of the cDNA insert.
The cDNA insert contains a single open reading frame.
As a control we transduced the retroviral vector without cDNA insert.
The cDNA insert was sequenced.
A clone including the longest cDNA insert was used for sequencing.
The cDNA insert of the resultant plasmid can be sequenced directly.
One of these phages was digested with EcoRI to remove the cDNA insert.
The cDNA insert is within BamHI and XbaI sites in the vector.
Isolated plasmids were digested with NsiI to verify orientation of the cDNA insert.
The parent vector without cDNA insert was used in the negative control transfections.
See also
The blot was probed sequentially with the indicated radioactively labeled cDNA insert.
The cDNA insert totals approximately 4 kilobasepairs.
Resultant colonies contained the double stranded phagemid vector with the cloned cDNA insert.
The cDNA insert from this colony was 2 kb in length.
DNA and deduced amino acid sequences for the cDNA insert are set out in SEQ.
One cDNA insert found this way is present in pML5.
DNA was generated by PCR amplification of the cDNA insert from clones.
The cDNA insert was separated from the phage vector arms by 1 % agarose gel electrophoresis.
This clone was isolated, and its cDNA insert was sequenced.
The ME-5 cDNA insert was ligated with the vector and competent bacteria transformed.
In this figure, a thick circle part corresponds to the cDNA insert.
Containing a human hippocampus cDNA insert encoding a cGS-PDE isozyme fragment.
The closed triangles indicate the positions of nucleotide substitutions in the cDNA insert of pCAM1.
The complete sequence of the cDNA insert is shown in Figure 3.
The cDNA insert pTM22 represents a genetic locus that is not a member of family IV.
From the positive clones clone pHIRF31 containing the longest cDNA insert was selected.
Colonies containing the IL-4 cDNA insert were selected using a labeled oligonucleotide probe.
From each individual colony, the activating plasmid was rescued and the cDNA insert was sequenced.
The EPP2 cDNA insert was ligated with the vector and competent bacteria transformed.
Containing a human glioblastoma cell cDNA insert encoding a RAS - related polypeptide ;.
The plasmid pCHRM1 was digested with Not I to release the cDNA insert.
Subclones of the H23 cDNA insert in pBLUKSPTM are analyzed by restriction analysis.
The restriction endonuclease map of the human u-PA cDNA insert is shown in Fig.
The purified OCIF4 cDNA insert was ligated using DNA ligation kit ver.
Sequence is then obtained from the complete GLP-2 receptor cDNA insert from a positive pool.
The cDNA insert in pTM22 encodes a novel human gene.
Figure 2B is a restriction endonuclease cleavage map of the cDNA insert of Figure 2A.
The cDNA insert of claim 6, wherein said cDNA insert is included in a plasmid.
The blot was hybridized with the cDNA insert of λ29.
The cDNA insert of plasmid pGRN121 was sequenced using techniques known in the art.
Figure 4 illustrates the nucleotide sequence of the cDNA insert of pH15C.
Containing a human hippocampus cDNA insert fractionally encoding a 61 kDa CaM-PDE isozyme ;.
Each plaque represents a clone of a unique λ gt10 phage containing a cDNA insert.
The cDNA insert of claim 6, wherein said cDNA insert is carried by a host cell.
Northern blots were hybridised with a radiolabelled ct-Bmy cDNA insert and autoradiographed ( upper panels ).
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The cDNA is divided into two portions
The orientation of bikunin cDNA was confirmed by sequencing
A cDNA was also isolated from a human library
