Examples of 'cdna fragment' in a sentence
Meaning of "cdna fragment"
cdna fragment - In molecular biology, a cDNA fragment refers to a small piece of complementary DNA (cDNA) that has been produced through reverse transcription of messenger RNA (mRNA). It is a copy of a specific mRNA sequence that can be used for various research purposes, such as gene expression analysis or cloning
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cdna fragment
The cDNA fragment is integrated into a plasmid vector.
Thus there was obtained a cDNA fragment having flush ends.
The PCR method was also exploited to clone cDNA fragment.
The said cDNA is the cDNA fragment of OsNACx gene.
Each HCV cDNA fragment was fractionated by agarose gel electrophoresis and purified.
The complete nucleotide sequence of the cDNA fragment was determined.
The actin cDNA fragment was purchased from Clontech.
Completion of the sequencing of a given extended cDNA fragment is assessed as follows.
The recovered cDNA fragment was ligated in the Eco RI site of λ phage.
The purified liner vector was ligated with the cDNA fragment and transformed into bacteria.
Translation of this protein was initiated at an internal ATG within the inserted cDNA fragment.
The complete nucleotide sequence of the cDNA fragment was determined as described above.
However not every All of the clones do not contain cDNA fragment.
PCR primers are developed and the cDNA fragment is isolated using PCR technology.
Screening of human BNP clone is carried out using a labeled porcine cDNA fragment as a probe.
See also
The transfer of the dengue cDNA fragment made use of this Xho I site.
This polylinker includes the Sma I site into which the CpTI cDNA fragment was inserted.
A cDNA fragment FIG.
To produce the plasmid a Gait cDNA fragment from pCMV.
The mutated cDNA fragment was then reintroduced into pT7SFP.
To produce the plasmid a Galt cDNA fragment from pCMV.
Kb cDNA fragment encoding human B-actin was used as the control probe.
To produce this plasmid the Gait cDNA fragment from pCMV.
The IL-5 cDNA fragment is inserted into an expression vector derived from baculovirus.
To produce this plasmid the Galt cDNA fragment from pCMV.
The cDNA fragment of BRO1 was therefore identified as encoding a cyclin binding protein.
Phage DNA was digested with EcoRI to release the cloned cDNA fragment for subsequent subcloning.
The cDNA fragment so obtained was cloned into vector UNI-ZAPXR as follows.
Isolation of human OBM cDNA fragment by PCR.
A cDNA fragment encoding human Factor IX was obtained and the 3 ' untranslated region was deleted.
After the primer was digested with HindIII, the cDNA fragment was ligated with the linker.
The cDNA fragment was isolated and purified using the Bresaclean kit ( Bresatec ).
The sequence of the cloned cDNA fragment is shown in Figure 8.
The xylanase cDNA fragment was isolated from one of the clones by cleavage with Hindlll / Xbal.
Thus, there was obtained a cDNA fragment coding for PAL.
A cDNA fragment encoding the thymosin B15 of the invention may readily be inserted into a suitable vector.
These oligonucleotides were used to amplify a cDNA fragment of the B-glucosidase gene by RT-PCR.
Figure 2D depicts the derived amino acid sequence of the senescence-induced Arabidopsis DHS cDNA fragment.
Subsequently, TCF-II cDNA fragment was inserted into pcDNA I fragment by the following reactions.
Northern blot analysis was performed using the radio-labeled DEG5 cDNA fragment as a probe.
The cDNA fragment was amplified by PCR using a primer set of T3 and T7 promoter sequences.
To produce this plasmid the Bglll / BamHl Galt cDNA fragment from pCMV.
For both plant species a 350 bp cDNA fragment was obtained which was cloned and sequenced.
This construction adds 9 codons to the coding sequence of the 39 cDNA fragment.
This allowed identification of a cDNA fragment comprising the following 171 nucleotide long sequence,.
Plaques were screened by hybridization to a radiolabelled probe generated from a subcloned band 200 cDNA fragment.
Similar results were obtained by using a cDNA fragment close to the 3 ' end.
A cDNA fragment of a 1270 bp encoding the above amino acids and heaving EcoRI termini was prepared.
A strong 150 bp band representing an amplified cDNA fragment was visualized.
The synthetic cDNA fragment contained NcoI and XbaI cohesive ends at 5 ' and 3 ' terminal respectively.
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Examples of using Cdna
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The cDNA is divided into two portions
The orientation of bikunin cDNA was confirmed by sequencing
A cDNA was also isolated from a human library
Examples of using Fragment
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He has a fragment of sword embedded in his chest
There is a tiny missing fragment on the occipital
A small fragment impinging on her spinal cord
