Examples of 'cell supernatants' in a sentence
Meaning of "cell supernatants"
refers to the fluid or liquid that remains after cells have been centrifuged or separated from the solid components, often used in laboratory or biological contexts
How to use "cell supernatants" in a sentence
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cell supernatants
Hybridoma cell supernatants were screened by two assays.
Collection of the cell supernatants.
Use of crude cell supernatants or complex cultivation mixtures.
The neutralizing activity can also be characterized by haemagglutination test on cell supernatants.
A not concentrated cell supernatants were used.
The cell supernatants were harvested and concentrated by crossflow filtration.
Virus particles can typically be recovered from cell supernatants or lysed cells.
Hybridoma cell supernatants containing IgGs are collected twice a week.
The mutant toxins can then be purified from host cells or host cell supernatants.
Antibodies in hybridoma cell supernatants may be used as said test compounds.
Cell supernatants containing antibody were harvested sequentially up to four times with subsequent media replacement.
Only minimal volumes of the cell supernatants containing each recombinant virus remained.
Proteins expressed using the methods described may be purified from cell lysates or cell supernatants.
The cell supernatants containing the desired antibody are identified by specific tests.
These DNA fragments were recovered from the cell supernatants.
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Components from the cell supernatants can be further purified using standard techniques.
Silver stain and Western blot analysis of hNGF purified from virus infected cell supernatants.
The collected aliquots of cell supernatants were assayed for the concentration of antibody present.
Determination of biological activity in COS cell supernatants.
Hybridoma cell supernatants are analysed in a direct ELISA.
These DNA fragments are recovered from cell supernatants.
LAK cell supernatants are obtained as follows.
The number of radioactivity in the cell supernatants was then determined using Topcount.
Cell supernatants containing newly formed viruses were harvested and titrated using MDCK cells.
After blocking, cell supernatants were added.
Cell supernatants were brought to pH 8 with NaOH.
Titers of one experiment ; concentrated cell supernatants were used.
Antibodies from the cell supernatants are then screened by ELISA for reactivity against the immunogen.
The results are expressed in pg / mL of leptin present in the cell supernatants.
TNFa levels in cell supernatants are quantified using a standard sandwich ELISA technique.
Rx, only the precursor prorelaxin immunoprecipitated from cell lysates or cell supernatants.
The T cell supernatants were collected and subjected to sandwich ELISA.
Hyaluronic acid was assayed in the cell supernatants as described in example 1.
Vero cell supernatants also exhibited HA-Titers.
Cell-derived human cytokine concentrations were determined in cell supernatants from each microplate.
Cell supernatants were analyzed for cytokine contents after 3 days of culture.
Figure 4 is a diagram showing increased binding of zearalenone by enzymatically treated yeast cell supernatants.
If desired, cell supernatants may be concentrated to raise the detection level.
Briefly, ten-fold serial dilutions of the transfected cell supernatants were prepared in infection medium.
Cell supernatants were removed and split into two groups of six,.
This is a new finding, and stresses that cell supernatants should be used in therapeutic regimens.
Infected cell supernatants were removed and 50 µl of lysis buffer was added per well.
Example 1 Samples of human plasma, urine and cell supernatants were analysed quantitatively.
If necessary, cell supernatants may be concentrated to raise the detection level.
The mAbdAbs set out in table 1 were expressed transiently in CHOK1 cell supernatants.
Twice a week, cell supernatants were collected, and drugs and medium were renewed.
Then, THF-alpha production was measured in the cell supernatants using ELISA.
Cell supernatants were analyzed by gelatin zymography after 48 h of incubation with the corresponding stimulus.
Cytokine and chemokine levels in cell supernatants were measured as described in Example 10.
T cell supernatants are measured for the presence of interleukin-2 by commercially available ELISA.
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Examples of using Supernatants
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The culture supernatants were screened as described above
The difference is very significant in the supernatants
The resulting supernatants were dried under vacuum
