Examples of 'cell supernatant' in a sentence
Meaning of "cell supernatant"
cell supernatant - Cell supernatant refers to the liquid or fluid portion of a sample that remains after the cells have been removed by centrifugation or sedimentation. It is commonly used in laboratory settings for various analyses or experiments
How to use "cell supernatant" in a sentence
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cell supernatant
The cell supernatant was then removed and discarded.
Carefully remove cell supernatant.
The cell supernatant was collected to determine IgG production.
The remainder of the filtered cell supernatant can be stored frozen.
The cell supernatant and wash supernatants were collected and pooled.
As a result the proteins are located in the periplasm or in the cell supernatant.
The presence of virus in cell supernatant was determined by hemagglutination assays.
The plates are centrifuged to allow the harvest of cell supernatant only.
Subsequently cell supernatant is collected and incubated with putative inhibitors.
The antisera were then immunoreacted with the transferred cell supernatant.
Baculovirus is then collected from cell supernatant and purified by plaque assay.
The cell supernatant or the cells themselves may be harvested for analysis.
Tubes were centrifuged and a dilution series of the cell supernatant was made.
The cell supernatant is carefully removed either by aspirating supernatant or by flicking the plate.
Explain how to purify and characterize exosomes from transfected cell supernatant.
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The peptide may be isolated from the cell supernatant if the peptide is secreted.
Cell supernatant was passaged once in MDCK cells and twice in chicken embryonated eggs.
It is necessary to perform the cell check and cell supernatant check extremely rapidly.
Each COS cell supernatant was tested in duplicate.
The recombinant product was purified from Pichia pastoris cell supernatant as described below.
Cell supernatant of the centrifuge tube was aspirated . f.
The nuclei were pelleted and the cell supernatant was digested with RNase and DNase.
Target cell killing was detected by measuring LDH release into the cell supernatant.
Infection of target cells by cell supernatant containing Lentiviral vector.
Negative controls were unstained cells with or without mock-transfected cell supernatant.
Plaques are precipitated from the cell supernatant with polyethylene glycol and resuspended in PBS.
Extraction of viral HBV DNA from cell supernatant.
Two days later, the cell supernatant was harvested and assayed.
Next, displacement experiments were conducted using purified antibody, rather than cell supernatant.
Otherwise, the cell supernatant or lysate is directly pre-purified using chromatographic methods.
The polypeptide may be recovered, for example, from cell lysate or from the cell supernatant.
In certain embodiments, the cell supernatant or cell lysate is harvested following the production phase.
In a more specific embodiment, the mammalian cell culture supernatant is a CHO cell supernatant.
Cell supernatant is harvested, and analyzed for cleavage fragments, for example using immunoassay.
Harvesting cells and cell supernatant comprising AAV particles ;.
Conclusion, Calcium has a stabilizing effect on FVIII in cell supernatant medium.
Otherwise, the cell supernatant or lysate is directly pre-purified using chromatographic methods as described hereinbefore.
Example 15 Stability of FVIII containing cell supernatant with different calcium concentration.
In the cell supernatant was measured using an ELISA-based FACS method.
The recombinant antibody was purified by Protein G affinity column ( PIERCE ) from the collected cell supernatant.
After six days, the cell supernatant was recovered and used for the protein purification.
No significant FasL immunoreactivity was seen with the control 293 cell supernatant sample.
Uninfected SF9 cell supernatant was included as a negative control.
Next, El-Andaloussi et al . explain how to purify and characterize exosomes from transfected cell supernatant.
Mock transfected cell supernatant with no recombinant ADAMTS 13 was also provided as a control.
After 48 hours, a fraction of the NHK cell supernatant was removed.
The sample is a cell supernatant or filtrate comprising the botulinum toxin A complex ; or.
The pellet was resuspended in 1.5 uL of TNE for each mL of cell supernatant.
The processed cell supernatant was batch loaded to the Amylose resin overnight, with rocking.
Virus yields were monitored 10 days after infection by a p24 Ag ELISA on the cell supernatant.
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A virus titration is performed on the harvested supernatant
The supernatant was carefully separated to a different tube
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I memorized the cell coverage in this area
Cell phone number include area code
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