Examples of 'chaotropic agent' in a sentence
Meaning of "chaotropic agent"
chaotropic agent: In science, a chaotropic agent disrupts the structure of molecules, particularly proteins and nucleic acids, by interfering with the hydrogen bonding and hydrophobic interactions. This disruption leads to the denaturation of the molecules
How to use "chaotropic agent" in a sentence
Basic
Advanced
chaotropic agent
A particularly preferred chaotropic agent is sodium perchloride.
The chaotropic agent will then be removed by dialysis.
It is preferred that the chaotropic agent is a chaotropic salt.
Urea is usually used as a weaker denaturant or chaotropic agent.
A preferred chaotropic agent is guanidinium thiocyanate.
An inhibitor removal buffer is characterized in that it contains a chaotropic agent.
An exemplary chaotropic agent is guanidine.
It thus has a dual action as a detergent and as a chaotropic agent.
He chaotropic agent includes an alcohol substance and a substrate solution.
The reagent comprises a metal salt and a chaotropic agent.
The chaotropic agent stabilizes nucleic acids by inhibiting nuclease activity.
It is very much preferred that the chaotropic agent is a chaotropic salt.
The chaotropic agent includes an alcohol substance and a substrate solution.
Solubilization can also be carried out at neutral pH by means of a chaotropic agent.
The chaotropic agent comprises an alcohol substance and a substrate solution.
See also
A third key ingredient of the buffer is an effective amount of a chaotropic agent.
Any buffer or chaotropic agent or stabilizer which binds calcium may be used.
This solution contains both a fixative and a chaotropic agent such as formamide.
A chaotropic agent is a chemical that can disrupt the hydrogen bonding structure of water.
This method uses an extraction buffer comprising a chaotropic agent in combination with a detergent.
A chaotropic agent is a substance that disrupts the structure of and denatures macromolecules such as proteins.
Known extraction techniques generally use a chaotropic agent such as guanidinium to lyse the cells.
The reagent is characterized in that the reagent comprises a metal salt and a chaotropic agent.
The concentration of chaotropic agent will directly affect its strength and effectiveness.
The extraction methods disclosed herein do not use a protein denaturing agent such as a chaotropic agent.
Addition of the chaotropic agent in these concentrations causes the pathogenic prion protein to denature.
Washing with washing buffer removes residual chaotropic agent from the bound nucleic acids.
The chaotropic agent is a chaotropic salt ;.
In some embodiments, the chaotropic agent is not urea.
In one embodiment, the sample is homogenized in the presence of a chaotropic agent.
Optionally, a chaotropic agent may be added.
The method according to claim 5, wherein the matrix comprises a chaotropic agent.
A preferred chaotropic agent is guanidine-HCl.
The method of claim 1 wherein the chaotropic agent is urea.
Preferably, the chaotropic agent in the third assay reagent is sodium perchlorate.
The method according to claim 1, wherein the chaotropic agent is sodium thiocyanate.
In particular, guanidinium hydrochloride and / or guanidinium thiocyanate can be used as chaotropic agent.
In one embodiment, the chaotropic agent is a guanidinium compound.
Homogenising a sample in an acidic denaturing composition comprising a chaotropic agent and phenol ;.
In some embodiment, the chaotropic agent is not guanidinium thiocyanate.
The preparation is solubilized in an aqueous solution containing a chaotropic agent or a detergent, or both.
In general, any buffer or chaotropic agent or stabilizer which binds calcium may be used.
The method of claim 9 wherein the cell dissociation solution comprises a chaotropic agent.
The method of claim 2 wherein said chaotropic agent is sodium perchlorate.
Typically the chaotropic agent may be guanidine hydrochloride, potassium thiocyanate or sodium chloride.
Process according to claim 8 in which the chaotropic agent is urea or guanidine-HCl.
Process according to claim 5 in which the insoluble aggregate is solubilized with a chaotropic agent.
The kit according to claim 1, wherein the chaotropic agent is guanidine thiocyanate.
A method according to claim 1, additionally comprising in step a contacting said tissue with a chaotropic agent.
Freeing nucleic acids is performed at a chaotropic agent concentration of at least 2M.
You'll also be interested in:
Examples of using Agent
Show more
Another good agent going down a bad path
Agent detection versus exposure host response
That might get agent sanburg a medal
Examples of using Chaotropic
Show more
A particularly preferred chaotropic agent is sodium perchloride
The chaotropic compounds are employed in conventional amounts
Nucleic acid binds to MGPs in chaotropic solution
