Examples of 'chaotropic agents' in a sentence
Meaning of "chaotropic agents"
Chaotropic agents: Substances that disrupt the structure of water molecules, commonly used in laboratory settings to separate molecules based on their hydrophobicity or interact with biomolecules in research experiments
How to use "chaotropic agents" in a sentence
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chaotropic agents
Exemplary chaotropic agents are guanidine and urea.
Certain organic solutes and chaotropic agents.
Chaotropic agents are a very important feature of the present invention.
Guanidine salt and urea are used frequently as chaotropic agents.
Significant amounts of chaotropic agents are needed to observe perceptible unfolding.
Lysis in the laboratory may be affected by enzymes or detergents or other chaotropic agents.
Representative chaotropic agents include urea and guanidinium hydrochloride.
Such denaturing agent may be selected from a group comprising detergents or chaotropic agents.
Chaotropic agents are commonly used as lysing agents in the extraction process.
Yields for methods employing chaotropic agents are about the same.
The chaotropic agents and detergents are commonly used as solubilizing agents.
In the presence of high concentrations of chaotropic agents the reaction is almost quantitative.
In general chaotropic agents increase the solubility of nonpolar substances in water.
Sodium thiocyanate can be replaced with ammonium thiocyanate and other chaotropic agents.
Representative examples of chaotropic agents are urea and its derivatives and guanidine.
See also
Chaotropic agents like guanidiunium salts can also act as lysing agents in this system.
The wash may also contain detergents and chaotropic agents to minimize nonspecific binding.
Chaotropic agents include for example urea, guanidine hydrochloride.
Besides a strong denaturing effect on proteins high concentrations of chaotropic agents also mediate cell lysis.
This kit uses chaotropic agents and detergents to lyse selectively mammalian cells.
In certain embodiments, guanidine hydrochloride or urea are the chaotropic agents.
However, other chaotropic agents can also be used.
Preferably, the proteolytic enzyme is active in presence of one or more chaotropic agents.
It is common to use chaotropic agents as e . g.
Examples of chaotropic agents are urea, guanidine hydrochloride and ammonium thiocyanate.
Thus, it is preferred that the reaction mixture is substantially free of chaotropic agents.
Chaotropic agents are generally used at very high concentrations, and are expensive.
The activation of MMPs can occur vía many mechanisms, these include chaotropic agents e . g.
Furthermore, irritant chaotropic agents are not necessary for the purification process.
Other methods for DNA isolation utilize non-corrosive chaotropic agents.
Examples of preferred chaotropic agents include urea, guanidine hydrochloride, arginine and sodium thiocyanide.
The protease is not active towards C1 proteins at neutral pH and 50°C without chaotropic agents.
Thus, such chaotropic agents are preferably not used for the methods of the present invention.
Molysis ™ from the company Molzym, uses chaotropic agents and detergents to lyse selectively mammalian cells.
Chaotropic agents include, guanidinium compounds, urea, formamide, potassium iodiode, potassium thiocyantate and similar compounds.
Other methods have involved other chaotropic agents such as sodium dodecyl sulphate ( SDS ) e . g.
Chaotropic agents are generally used at concentrations in the range of 1 to 9M.
Unfolding of Tn3 by chaotropic agents was monitored by intrinsic fluorescence.
Suitable chaotropic agents include, for example, guanidinium salts.
Representative chaotropic agents include urea, guanidine-HCl, and sodium thiocyanate.
Examples of chaotropic agents include sodium dodecyl sulfate ( SDS ) and urea.
Suitable chaotropic agents include urea, sodium bromide, guanidine hydrochloride, KCNS, potassium iodide and potassium bromide.
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Examples of using Chaotropic
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A particularly preferred chaotropic agent is sodium perchloride
The chaotropic compounds are employed in conventional amounts
Nucleic acid binds to MGPs in chaotropic solution
