Examples of 'cre recombinase' in a sentence
Meaning of "cre recombinase"
cre recombinase - Cre recombinase is a type of enzyme commonly used in genetic research to manipulate DNA sequences in organisms
How to use "cre recombinase" in a sentence
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cre recombinase
After integration of a construct the KanMX marker was removed with the cre recombinase.
The Cre recombinase is known in the art.
The recombinase may be a Cre recombinase.
A Cre recombinase targets the loxP sites for recombination.
Homozygous disruptants are transfected with a Cre recombinase expressing plasmid.
The expression of the Cre recombinase is detected by means of Western blot and immunodetection.
Selected clones were electroporated with a Cre recombinase expressing vector.
Cre recombinase has the capacity to split DNA regions flanked by loxP type sequences.
A lot of studies focussed on the Cre recombinase to explore its evolvability.
The selectable marker was then excised using the Cre recombinase.
A lot of studies focused on the Cre recombinase to explore its evolvability.
The cell may comprise a gene capable of expressing a Cre recombinase.
Cre recombinase or FLP recombinase are preferred recombinases.
The marker can be removed after integration by expressing Cre recombinase.
Recognized by a Cre recombinase enzyme.
See also
These mice carry a lacZ reporter that can be activated by Cre recombinase.
Cre recombinase will recombine the lox sites and remove the drug-resistance genes.
The recombination sequences to which the Cre recombinase binds are named loxP.
The hygromycin cassette may optionally be removed by breeding to mice that express Cre recombinase.
Cre recombinase catalyzes the site-specific recombination of an intervening target sequence located between loxP sequences.
Said transgenes are removed by expressing the Cre recombinase in the beta cells.
For example, the Cre recombinase specifically binds a loxp recombination site, and thereby induces recombination.
The supercoiled plasmids were combined with lambda Integrase and Cre recombinase in vitro.
Preferably, the recombinase is Cre recombinase and the recombinase target sites are loxP sites.
In a specific embodiment, the recombinase is a Cre recombinase.
A similar system has been developed using the Cre recombinase / loxP recognition sites of bacteriophage P1.
Preferably, said sequences are LoxP sequences of the Cre recombinase.
Preferably, said recombinase is Cre recombinase or a variant thereof.
In one embodiment, the cell comprises a gene capable of expressing a Cre recombinase.
LoxP, Cre recombinase target recognition site sequence.
Figure 7 illustrates the evaluation of diversity in bacteria expressing cre recombinase infected by multiple phagemids.
In the examples with Cre recombinase we used an omega leader of TMV U1.
In some embodiments, the recombinase is a Cre recombinase.
Cre, The Cre recombinase is a site-specific recombinase.
In another embodiment, the recombinase is Cre recombinase.
The URA3ex marker used can be excised via Cre recombinase expression.
The recombination reaction is catalyzed by enzymes known as recombinases, such as Cre recombinase.
Such an activating gene may be a gene encoding a so-called Cre recombinase or Cre-like protein.
The targeting vector of claim 19 wherein said sequences are LoxP sequences of Cre recombinase.
Advantageously, a plasmid carrying the gene coding cre recombinase will be used Sauer, B.
In turn, the RheoSwitch ® inducible promoter controls expression of the Cre recombinase.
The efficacy of tamoxifen treatment to induce Cre recombinase was tested at P20.
Accordingly, genetic recombination of the target sequence is dependent on expression of the Cre recombinase.
In the same manner, where lox sites are utilized, the Cre recombinase is required.
The site-specific recombination sequences are advantageously loxP sequences ( SEQ ID NO, 5 ) of Cre recombinase.
Escherichia coli LoxP recombination site, recognized by a Cre recombinase enzyme.
In a further embodiment, the host cell comprises a gene capable of expressing a Cre recombinase.
Such cryptic sites have been described for the Cre recombinase ( 38 ).
In one embodiment, the SRRS sites are recognizable by a Cre recombinase.
In one embodiment, the RSSR sites are recognizable by a Cre recombinase.
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