Examples of 'recombinase' in a sentence
Meaning of "recombinase"
Recombinase: An enzyme that catalyzes the process of genetic recombination in cells
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- Any of several enzymes that mediate recombination of DNA fragments among chromosomes
How to use "recombinase" in a sentence
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recombinase
Kit for multiplexing recombinase polymerase amplification.
Recombinase activity comprises a number of different functions.
Methods for multiplexing recombinase polymerase amplification.
The recombinase may be transiently expressed or provided.
This enzyme can be a transposase or a sitespecific recombinase.
Beta recombinase promotes recombination in chromatin structures.
In some embodiments the recombinase is a transposase.
The recombinase may be a recombinase as set forth herein.
Methods of generating such recombinase variants are described herein.
Inducing expression of the recombinase.
Expression of the altered recombinase is typically desired to be transient.
Probes may be chosen as suited to the recombinase chosen.
The recombinase agent is RecA protein.
Using family shuffling or recombinase chain reaction methods.
Also described herein is a method for identifying an altered recombinase.
See also
Assay for transient recombinase activity in HeLa cells.
A recombination site is a nucleic acid sequence specifically recognized by a recombinase.
Two different approaches of recombinase delivery are exemplified in the invention.
The cell can endogenously or recombinantly express the recombinase.
Use of a tailored recombinase for the treatment of retroviral infections.
The cell may comprise a gene capable of expressing a recombinase.
It is therefore desirable to express the recombinase activity only transiently.
Also disclosed are integrating enzymes wherein the enzyme is a recombinase.
Linkage is performed by adding the appropriate recombinase to facilitate recombination.
Said recombinase can be operably linked to either a constitutive or an inducible promoter.
Transciption stop signals flanking the recombinase are also desirable.
The recombinase may be under the control of a constitutive or inducible promoter.
This can involve one or more recombinase recognition sites.
The bacterial cell is then induced to express the recombinase.
The modifying portion is preferably not a recombinase or a restriction endonuclease.
The altered recombinase may be introduced into the target cell as a polypeptide.
The RTSs are exposed to or contacted with a recombinase in any number of ways.
Tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains.
In a preferred embodiment the tailored recombinase protein is linked to a second polypeptide.
The target sequence is amplified with the help of recombinase agents.
Thee polypeptide possessing recombinase may be a recombinase as set forth herein.
The doubly mutated target sites are not well recognized by the recombinase.
Preferably expression of the recombinase is under the control of an inducible promoter.
This results in stable integration with little or no recombinase mediated excision.
Described herein is an altered recombinase produced by the methods of the present invention.
This is one factor contributing to tolerability of the tailored recombinase in human cells.
Nucleic acids encoding a tailored recombinase defined by a sequence are also provided herein.
Said recombinase can be delivered in cis together with one of said components.
Each recombinable unit comprises one or more internal recombinase recognition sites.
The recombinase enzyme catalyses the recombination reaction between two recombination sites.
Inserted vector backbone sequences are subsequently removed by the action of an appropriate recombinase.
An example is a recombinase.
The recombinase may be a recombinase that is not naturally expressed in the cell or embryo.
The repressor is expressed constitutively and represses the expression of the recombinase.
An appropriate recombinase is provided and the transfer cassette is integrated at the target site.
