Examples of 'crude lysate' in a sentence
Meaning of "crude lysate"
crude lysate: Crude lysate refers to a biological sample that contains a mixture of various cellular components obtained by breaking open cells. This term is commonly used in scientific research, especially in biochemistry and molecular biology, to describe the initial cellular extract before further purification or analysis
How to use "crude lysate" in a sentence
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crude lysate
Production of a crude lysate possessing enzymatic activity.
Activity was measured on the crude lysate.
The crude lysate was spun to remove cellular debris.
Activity is shown as units per mg of total protein in the crude lysate.
Crude lysate were then stored on ice prior to assay.
No activity was detected on the crude lysate corresponding to the native nucleic sequence.
The crude lysate obtained this way was used to plaque purify the mixture of viruses.
Stimulation of these cells with a Leishmania crude lysate yielded a mixed Th cytokine profile.
This crude lysate was to be used for the reaction.
Coomassie staining of a PAGE gel containing the crude lysate demonstrates that the scaffold is present.
This crude lysate thereby obtained may be used directly.
The column was loaded with the crude lysate at a flow rate of 8 ml per minute.
The crude lysate was further purified by DEAE-Sepharose chromatography.
Lane 1 represents the crude lysate prior to heat treatment.
Typically, yeast cells are treated by mechanical disruption using glass beads to produce a crude lysate.
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DNA in the crude lysate was degraded by sonication.
Thus, the polypeptides are usually first obtained as crude lysate of the host cells.
Centrifuging the crude lysate to yield a pellet and a supernatant ;.
Thus, the antibody is usually first obtained as crude lysate of the host cells.
Providing a crude lysate by a process according to any previous claim ;.
Lane 2 represents the soluble protein remaining after lowering the pH of the crude lysate to 3.0.
Lane 1 represents the crude lysate prior to protease treatment.
The crude lysate is amplified ( primary amplification ) to obtain a working stock of zvegf4 rAdV lysate.
After 30 minutes, the pressure was quickly released to disrupt the tissue and produce a crude lysate.
Preferably, the crude lysate is centrifuged, and the supernatant fraction is recovered.
B -- Demonstration of the fragmentation produced by the crude lysate.
Following solubilisation, the crude lysate is incubated at different temperatures for a set amount of time.
GFP expression was confirmed by fluorescent microscopy of A549 cells infected with the crude lysate.
Analysis of a crude lysate preparation resulting from a transfection of pAdApt35 . LacZ and the pWE.
A pump for primary filtration 112 delivers the crude lysate solution through a primary filter 113.
Crude lysate was recovered and filtered as before, yielding 85 L of clarified lysate.
Step A -- Production of a crude lysate possessing enzymatic activity.
The crude lysate was agitated at setting 5 in an incubator at 36°C for one hour.
A -- Preparation of a crude lysate.
This 100 µL crude lysate was to be used for the reaction.
Transfer of the crude lysate ( specimen extract ) to the Reaction Mix ( RM ).
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The clear lysate was deposited on the column
A preferred sample according to the invention is a lysate
Production of a crude lysate possessing enzymatic activity
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