Examples of 'cytosines' in a sentence
Meaning of "cytosines"
Cytosine is one of the four main bases found in DNA and RNA, and pairs with guanine
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- plural of cytosine
How to use "cytosines" in a sentence
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cytosines
Methylated cytosines are unaffected by the bisulfite reaction.
The number of guanines equals the number of cytosines.
The cytosines that have not converted in uracil are methylated.
It is therewith possible to differentiate between methylated and unmethylated cytosines.
The preserved cytosines provide the above said advantages.
It is therewith possible to differentiate between methylated und unmethylated cytosines.
Deamination of sulfonated cytosines takes place spontaneously in aqueous solution.
This example describes the use of an oligonucleotide bearing methylated cytosines.
Methylation density is determined for cytosines only or adenosines only.
The recovered cytosines among the CpG dinucleotides were scored as methylated.
It contains an equal number of thymines and cytosines and a single guanine.
Methylated cytosines in the promoter regions of a gene lead to its inactivation.
Preferably the presence or absence of methylation of one or more cytosines is analyzed.
All unmethylated cytosines are dominated in a bisulfite reaction and converted to uracils.
Methylation arrays are a traditional technique for assessing the methylation status of cytosines.
See also
Cytosines can be chemically modified so that they will not base pair with guanines.
Underneath is shown the sequence of the derived strand after conversion of cytosines to uracil.
Bisulfite treatment chemically converts unmethylated cytosines into a uracil while leaving methylated cytosines unmodified.
Sodium bisulfite is used in the analysis of the methylation status of cytosines in DNA.
Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA.
The converted DNA is then used for the detection of methylated cytosines.
The cytosines that were methylated were not limited to CG or CNG sequences.
Chemical treatment of DNA with sodium bisulfite converts unmethylated cytosines into uracils.
This study assessed expression of cytosines in mouse lungs after SEQ ID No.
The DNA sample then underwent bisulfite modification which converted unmethylated cytosines to uracil.
The cytosines to be analyzed thereby can be co-methylated or not.
A comparison of sodium bisulfate treated and untreated DNA provides for the detection of methylated cytosines.
After sequencing, the unmethylated cytosines appear as thymines.
Schiller and his team have developed micro-encapsulated cytosines.
However, methylated cytosines are unaffected.
The DNA is modified by sodium bisulfite treatment converting unmethylated Cytosines to Uracil.
For example, methylated cytosines may be substituted for cytosines.
In one embodiment, the chemical modification can be a conversion of cytosines to uracils.
Thereby non-methylated cytosines are converted to uracil while methylated cytosines remain unchanged.
This makes the discrimination between methylated and non-methylated cytosines possible.
In the sample nucleotide sequence, modified cytosines are not distinguished from cytosine.
Addition of a methyl group to the DNA, and this happens at cytosines.
Photochemical transition is performed, converting 5-methylcytosines to thymines but leaving unmodified cytosines unaffected.
In this method, bisulfite converts unmethylated cytosine bases to uracil, whereas methylated cytosines remain unaltered.
However, over time methylated cytosines tend to turn into thymines because of spontaneous deamination.
The target may be bisulfite-treated and non-methylated cytosines converted to uracil.
Following this treatment, unmethylated cytosines were converted to uracil and methylated cytosines remained unchanged.
Bisulfite treatment modifies DNA converting unmethylated, but not methylated, cytosines to uracil.
DNA can be methylated in cytosines and also in the proteins surrounding it, the histones.
Table 1 shows sequencing outcomes for cytosine and modified cytosines subjected to various treatments.
Subsequent PCR amplification replaces the uracil residues with thymines and the 5methylcytosine residues with cytosines.
The plasmid control indicated a full conversion of cytosines in bisulfite reaction ( empty circles ).
However, in certain embodiments the ISS may contain one or more methylated cytosines.
Approximately 60 % of cytosines located in CpG pairs are methylated.
DNA methylation occurs at CpG sites, and a majority of CpG cytosines are methylated in mammals.
