Examples of 'cytosine deaminase' in a sentence
Meaning of "cytosine deaminase"
cytosine deaminase: This phrase is a scientific term referring to an enzyme that catalyzes the removal of an amino group from the organic compound cytosine, often used in genetic engineering and medical research
How to use "cytosine deaminase" in a sentence
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cytosine deaminase
Cytosine deaminase is absent in plants.
An example of a prodrug activating gene is the cytosine deaminase gene.
The modified cytosine deaminase gene was then cloned into pLXSN.
The heterologous domain can comprise a cytosine deaminase of the disclosure.
The cytosine deaminase gene is found and expressed in a variety of microorganisms.
Additional modifications were made to enhance the stability of the cytosine deaminase.
Incorporation of the cytosine deaminase gene into the genome was demonstrated in two ways.
These cell populations was assayed for expression of the cytosine deaminase gene.
In such instances the cytosine deaminase is produced only in tumor cells following viral replication.
Any type of promoter can be operably linked to a nucleic acid sequence encoding a cytosine deaminase.
Various cytosine deaminase genes are available including those of bacterial origin and of yeast origin.
Intracellular HIV activity would then result in activation of the cytosine deaminase gene.
The codA gene encodes the enzyme cytosine deaminase which enzyme converts cytosine to uracil.
This assay therefore provides a robust measure of intrinsic DNA cytosine deaminase activity.
The bacterial gene for cytosine deaminase has recently been isolated and cloned I.
See also
The modified gene expression system would express cytosine deaminase and HIV.
For example, cytosine deaminase is absent in plants.
Technical Field The present invention relates generally to the enzyme cytosine deaminase.
The cytosine deaminase of claim 7 isolated from yeast.
Preferably, the prodrug activating protein is a cytosine deaminase enzyme.
CodA,, upp is a fusión between a cytosine deaminase gene and an uracil phosphoribosyl transferease gene.
In another embodiment, the negative selective marker is cytosine deaminase.
Cytosine deaminase converts 5-fluorocytosine to 5 fluorouracil, a potent antitumor agent.
Figure 5 illustrates that both fusión proteins exhibit cytosine deaminase activity.
When cytosine deaminase is the negative selective marker, a preferred interaction agent is 5-fluorocytosine.
An infective virion according to claim 7, wherein the heterologous enzyme is cytosine deaminase.
The resulting plasmid with the altered cytosine deaminase sequence is called pCD2.
The cell further can include a nucleic acid encoding the non-porcine cytosine deaminase.
A similar strategy uses the bacterial cytosine deaminase gene for conversion of 5-fluorouracil to 5-fluorocytosine.
The expression vector according to paragraph 7, wherein the desired gene is a cytosine deaminase gene.
A gene encoding cytosine deaminase ( CD ) is one example of the pro-drug-converting enzymes.
Another example of such a molecular safety switch is the cytosine deaminase ( CD ) gene.
The cytosine deaminase of any organism, for example E. coli or Saccharomyces cerevisiae, may be used.
Example 4 . Retrovirus mediated gene transfer results in successful expression of the cytosine deaminase gene.
Cytosine deaminase converts 5-fluorocytosine to 5-fluorouracil, which is highly cytotoxic.
Table 5 5FC selectively eliminates cells expressing the cytosine deaminase gene from mixed populations in vitro.
Suicide genes also include as non limiting examples caspase-9 or caspase-8 or cytosine deaminase.
The cytosine deaminase gene, when introduced into insect cells, confers sensitivity to the pro-drug 5-FC.
Preparation of a construct encoding E. coli cytosine deaminase.
Mammalian cells do not express significant amounts of cytosine deaminase and do not deaminate 5FC A.
Other enzymes include penicillin V amidase, penicillin G amidase and cytosine deaminase.
The oHSV of claim 19, wherein the transgene encodes cytosine deaminase or thymidine kinase.
Suitable negative selection markers include hsv - tk, hprt, gpt, and cytosine deaminase.
The mammalian host cell of claim 3, that expresses cytosine deaminase protein.
A method according to claim 10 wherein said gene encodes cytosine deaminase.
A construct as claimed in claim 6, wherein the enzyme is cytosine deaminase.
Example 4, Preparation of hMSC expressing cytosine deaminase.
Example 2, Construction of a retroviral vector expressing cytosine deaminase.
The suicide gene used in the present invention is cytosine deaminase ( CD ).
Inhibition of 3H-thymidine uptake by 5FC in lines expressing the cytosine deaminase gene.
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Examples of using Cytosine
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It binds to cytosine through three hydrogen bonds
Guanine will only bond with cytosine
Cytosine can also be deaminated to form uracil
Examples of using Deaminase
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Deaminase activity of both fusión proteins were examined
It is characterized by a deficiency of the enzyme porphobilinogen deaminase
Inhibition of deaminase increased intracellular araA
