Examples of 'degenerate primers' in a sentence
Meaning of "degenerate primers"
degenerate primers - in genetics or molecular biology, short single-stranded DNA sequences used to initiate DNA synthesis in PCR
How to use "degenerate primers" in a sentence
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degenerate primers
Two pairs of degenerate primers were made.
Internal fragments of genes were amplified using degenerate primers.
Sometimes degenerate primers are used.
Such primers are suitably degenerate primers.
The individual degenerate primers were used in hybridization mutagenesis reactions.
Conditions were similar to those used for the degenerate primers.
Degenerate primers are underlined.
The boxed amino acid sequences were used to develop degenerate primers.
Degenerate primers were used to allow for differences in the cyanobacterial codon usage.
Nondegenerate oligos can optionally be used in combination with degenerate primers disclosed.
Amplifications using degenerate primers can sometimes be problematic and may require optimization.
The obtained sequence of amino acid residues was used to design degenerate primers.
Amplification of these genes by pcr using degenerate primers was highly nonspecific in both samples.
The genes encoding the antibody can be cloned using degenerate primers.
The other use for degenerate primers is when primer design is based on protein sequence.
See also
The two arrows indicate the binding location of the two degenerate primers.
Use of degenerate primers can greatly reduce the specificity of the PCR amplification.
This is achieved by targeting highly conserved amino acid regions and by using degenerate primers.
Degenerate primers were then used in PCR processes to amplify chromosomal DNA from Taf.
The substitution was achieved by PCR using degenerate primers.
The sequences of degenerate primers were as follows,.
A number of HPV types were amplified by utilizing a mixture of specific and degenerate primers.
If desired, degenerate primers can be used for amplification.
All three were characterized by PCR using the degenerate primers described above.
In embodiments where degenerate primers are used, the resulting amplicons are degenerate.
A probe for identifying the Synechocystis AHAS gene was generated by PCR with degenerate primers.
Methods of making degenerate primers are well-known in the art.
The probe may be a large fragment, or one or more short degenerate primers.
In the present example, degenerate primers were designed to detect homeobox sequences.
The degeneracy code given on page 43 is used for the synthesis of the degenerate primers.
The following degenerate primers were designed and used in various combinations,.
Additionally, trinucleotides are useful in preparing degenerate primers for the polymerase chain reaction.
Degenerate primers were employed in multiple rounds of nested reactions to obtain the 3 ' end.
Are appended to the 5 ' end of the degenerate primers and are highlighted in uppercase letters.
The degenerate primers were directed towards the following regions / amino acid sequences,.
In this invention, the mixed primers were used for degenerate primers to clone above gene.
In certain embodiments, degenerate primers can be used as probes for non-human genetic libraries.
The genes encoding the antibody can be cloned using specific and / or degenerate primers.
Degenerate primers containing EcoRI and KpnI sites and further cloned into pUC 18.
Examples of specific degenerate primers are set forth in Figure 1.
Amplification, cloning and sequencing of DNA amplified with degenerate primers.
In particular, highly degenerate primers designed from these sequences are useful for this purpose.
Sequences shown were compared and used to design degenerate primers as described in EXAMPLE 2.
The degenerate primers used in this Example were as follows,.
PCR reactions were performed using degenerate primers applied to multiple single-stranded cDNAs.
The degenerate primers used for virus amplification are listed in Table 1.
As shown in Figure 2 three bands resulted from amplification with the degenerate primers.
However, amplifications using degenerate primers can sometimes be problematic and may require optimization.
Signal 3, Detection of DNA of different strains in a PCR reaction using degenerate primers.
Degenerate primers were designed to recognize conserved domains in the TGF-B dpp subfamily.
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Examples of using Degenerate
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They are soft and degenerate all through
As a degenerate orbit this is a radial elliptic trajectory
Then you will degenerate still more
