Examples of 'denaturing conditions' in a sentence
Meaning of "denaturing conditions"
denaturing conditions - conditions under which a substance loses its natural qualities or properties
How to use "denaturing conditions" in a sentence
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denaturing conditions
Denaturing conditions are well known in the art.
Miniaturized horizontal electrophoresis under denaturing conditions.
Isothermal denaturing conditions refers to conditions effective to denature a target molecule at a fixed temperature.
Isoelectrofocusing of proteins in a pH gradient and denaturing conditions.
RNAs chemically modified under denaturing conditions were selected for Rev binding through filter partitioning.
This is typically accomplished by use of appropriate denaturing conditions.
The samples were boiled under denaturing conditions and subsequently analyzed by SDS PAGE.
Immunocomplexes were analyzed by polyacrylamide gel electrophoresis under denaturing conditions.
Polypeptides may be prepared natively or under denaturing conditions and then subsequently refolded.
The oligonucleotides were purified by polyacrylamide gel electrophoresis under denaturing conditions.
Polypeptides may be prepared under native or denaturing conditions and then subsequently refolded.
Western analyses were performed to determine epitope disruptions under denaturing conditions.
Conveniently, gel filtration is employed under denaturing conditions followed by reversed phase chromatography.
Tryptic digestion of proteins in solution can be performed under native or denaturing conditions.
The denaturing conditions of the electrophoresis dissemble of particles into their constituent subunits, HBc monomers.
See also
These nucleic acid strands exist as a duplex until denaturing conditions are applied.
His-tag purification under denaturing conditions including refolding of the repeat proteins in the purification column.
Primary polynucleotide fragments were observed after gel electrophoresis under denaturing conditions.
The beads may then be washed under denaturing conditions to remove any non-covalently bound target.
Binding may be carried out with samples under native or denaturing conditions.
Electrophoresis was conducted under denaturing conditions with 10 % polyacrylamide gels.
The method according to the invention is favourably performed under completely denaturing conditions.
The 2-D gel analysis was performed under denaturing conditions in the presence of urea Fig.
Cell extracts may be analysed by polyacrylamide gel electrophoresis run under denaturing conditions.
Insulin, for example is sensitive to the denaturing conditions of the gastro-intestinal ( GI ) tract.
The proteins in the supernatant and the pellet are analysed by electrophoresis under denaturing conditions.
The cell pellet was resuspended under denaturing conditions in 5 ml 6 M guanidinium hydrochloride for 1 hour.
Native and nonnative protein states often coexist at equilibrium under mildly denaturing conditions.
Hybridization under partial denaturing conditions is further exemplified by the examples, including FIG . 5.
The extension products were analyzed by gel electrophoresis under denaturing conditions followed by autoradiography.
According to yet a further aspect there is provided a method of stabilizing a protein against denaturing conditions.
SDS PAGE was performed under denaturing conditions.
The total proteins of the bacteria thus cultured are separated by PAGE under denaturing conditions.
OmpT is heat stable and active even under denaturing conditions White et al ., ibid.
Furthermore, the separations can be conducted under native or denaturing conditions.
Dextran or heparin was added to TFPI under denaturing conditions in 6 to 8 M urea.
Cleavage products were resolved on 8 % polyacrylamide gels run under denaturing conditions.
The extension products were analyzed on 10 % polyacrylamide gels run under denaturing conditions followed by autoradiography.
The RNA was size resolved by electrophoresis through a 1 % agarose gel under strongly denaturing conditions.
After ligation, the sample is subjected to denaturing conditions Fig.
In some embodiments, the first plurality of oligonucleotides is released under denaturing conditions.
Proteins were recovered from inclusion bodies under denaturing conditions ( 8 M urea ).
Accordingly, in a preferred embodiment, the hybridization mixture is formed under denaturing conditions.
The DNA was resolved on a polyacrylamide gel under denaturing conditions.
In one embodiment, said conditions of step f comprise denaturing conditions.
In one embodiment, the protein of the present teaching is purified under denaturing conditions.
Preferably, the domain antibodies are exposed to the reactive groups under denaturing conditions.
In a preferred embodiment, the joining region remains intact under the denaturing conditions.
The variant is removed by affinity binding to a thiol-Sepharose column under denaturing conditions.
Single-stranded polymer may be obtained by gel purifying the product under denaturing conditions.
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Examples of using Denaturing
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It has been used as a denaturing and warning agent
The denaturing agent is advantageously a urea or a guanidine
Provisions regarding denaturing and binding agents
Examples of using Conditions
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There are no conditions or costs attached to this service
This sector is characterised by poor working conditions
Conditions and modalities for reimbursement or payment
