Examples of 'denaturing gel' in a sentence
Meaning of "denaturing gel"
denaturing gel ~ is a gel used in gel electrophoresis that contains chemicals to unravel DNA or protein molecules for better separation
How to use "denaturing gel" in a sentence
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denaturing gel
Integrity is checked by denaturing gel electrophoresis.
Denaturing gel electrophoresis.
Circularized intron is isolated by preparative denaturing gel electrophoresis.
A gradient denaturing gel runs overnight and requires about a day of set up time.
Fluorescein labeled oligonucleotides were purified by denaturing gel electrophoresis.
These are then subject to denaturing gel electrophoresis and autoradiography.
Nucleotides were ammonium deprotected and purified by denaturing gel electrophoresis.
Protected probe was quantified by denaturing gel electrophoresis followed by analysis on a Molecular Dynamics PhosphorImager.
A quenching agent is added and the products are analyzed suing denaturing gel electrophoresis.
RNA is subjected to denaturing gel electrophoresis and transferred to nitrocellulose or other support matrix.
Aliquots of reactions were withdrawn at indicated time points and analyzed by denaturing gel electrophoresis.
Figure 2 shows a denaturing gel of a single thrombin-binding peptide grafted on a cow antibody scaffold polypeptide.
The mRNA integrity was then determined by both spectroscopy and formamide denaturing gel electrophoresis.
Denaturing gel electrophoresis ( SDS-PAGE ) showed the isolated protein to be greater than 95 % pure.
RNA quality was assessed by denaturing gel electrophoresis.
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The concentration of RNA was measured and quality was check on a denaturing gel.
Proteins were then separated on a denaturing gel and analyzed by Western blot.
Alicruots of diluted reaction mixtures were used for TCA precipitation and alkaline denaturing gel analysis.
Equal amounts of Lysate were subjected to denaturing gel electrophoresis and Western blotting.
The pre-crRNAs were transcribed in vitro with T7 polymerase and purified on a denaturing gel.
RNA was isolated and analyzed by denaturing gel electrophoresis.
The term “ apparent molecular weight ” refers, as usual, to the molecular weight on a denaturing gel.
To estimate the length of the individual strands, denaturing gel electrophoresis was performed.
Microsomal or total extracted proteins from the treated cells were separated on a 7.5 % SDS-polyacrylamide denaturing gel.
Fig . 6 is a diagrammatic representation of an example denaturing gel used in mismatch detection.
The oligomers are then purified on 20 % polyacrylamide denaturing gel.
Twenty-five microliters are electrophoresed over a 6 % denaturing gel and the gel is exposed to autoradiographic film.
The resulting products were displayed on a 6 % acrylamide denaturing gel.
The fractions were also analyzed by denaturing gel electrophoresis ( inset ).
These samples are subjected to electrophoresis ( SDS-PAGE ) on denaturing gel.
FIG . 21 shows microbial diversity analysis using denaturing gel gradient electrophoresis ( DGGE ; Trial 1 ).
The quality of the RNA was verified by electrophoresis on a 1.5 % agarose denaturing gel.
When purified, these run as a single fragment on denaturing gel electrophoresis gels ( Figure 7 ).
This figure shows the imaging results of 6 % denaturing gel.
The amplification products were analyzed on a 7 % polyacrylamide denaturing gel and then revealed by autoradiography.
The integrity and quantity of RNA transcripts were further checked using denaturing gel electrophoresis.
RNA recovered from reactions was analyzed by denaturing gel electrophoresis.
After incubation, a 5 µl aliquot of each reaction mixture was analyzed by denaturing gel electrophoresis.
Digestion of the RNA substrate was analyzed by denaturing gel electrophoresis.
Products were analyzed by TCA ( 10 % trichloroacetic acid ) precipitation and / or denaturing gel electrophoresis.
The integrity of the total RNA was verified by denaturing gel electrophoresis.
The proteins translated in vitro were then electrophoretically separated in a denaturing gel ( FIG . 13, Panel A ).
The reaction products were analyzed following electrophoresis through a polyacrylamide-urea denaturing gel as described in Example 2.
The RNA-DNA hybrids are digested with ribonuclease A and analyzed using denaturing gel electrophoresis.
Figure 2 shows the 18S and 28S RNA bands after separation by denaturing gel electrophoresis.
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Examples of using Denaturing
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It has been used as a denaturing and warning agent
The denaturing agent is advantageously a urea or a guanidine
Provisions regarding denaturing and binding agents
Examples of using Gel
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The gel pads need to be changed periodically
Colourful silicone gel design to the front
Gel is essential when things get complicated
