Examples of 'dideoxy' in a sentence
Meaning of "dideoxy"
Dideoxy is a term used in genetics to describe a method of DNA sequencing that uses modified nucleotides lacking a 3'-OH group
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- Lacking two hydroxyl groups.
How to use "dideoxy" in a sentence
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dideoxy
Several clones were sequenced by the dideoxy method.
The dideoxy sequencing method was used.
This invention relates to dideoxy nucleoside analogs.
Use of dideoxy nucleoside analogues in the treatment of viral infections.
A positive clone is sequenced by the dideoxy method.
Dideoxy sequence analysis was performed following polymerase chain reaction amplification.
The larger isolate was sequenced by dideoxy sequencing.
The dideoxy method was eventually used to sequence the entire human genome.
Clones were confirmed by dideoxy termination sequencing.
The amplification products were sequenced directly by the dideoxy technique.
Preferred oligonucleotides are dideoxy based and are provided below.
Clones were sequenced on both strands by the conventional dideoxy method.
The sequences were verified by dideoxy chain termination sequencing.
Many improvements are oriented towards several steps of the dideoxy method.
Clones are confirmed by dideoxy termination sequencing.
See also
The mutated inserts were sequenced by the dideoxy method.
Positive clones were subjected to dideoxy sequencing to confirm the mutagenesis.
The specificity of each product was confirmed by dideoxy sequencing.
The example above describes use of a dideoxy nucleotide that is removed by pyrophosphorolysis.
The presence of the mutation is confirmed by dideoxy sequencing.
Samples suitable for dideoxy sequencing by capillary gel electrophoresis were prepared as follows.
All sequences were confirmed by dideoxy sequencing.
This method utilizes a dideoxy terminator in a Sanger sequencing reation.
Mutant sequences were confirmed by dideoxy sequencing.
Among the disadvantages of the dideoxy method is the expense associated with making ddNTPs.
The identity of the construct was confirmed by dideoxy sequencing.
It was sequenced by the dideoxy chain termination method of Sanger et al Proc.
Mutagenized phage clones were analyzed by dideoxy sequence analysis.
The sequence was determined by dideoxy sequencing of overlapping deletions generated by exonuclease III digestion.
The nucleic acid may be sequenced by dideoxy or other methods.
Four standard dideoxy DNA sequencing reactions were performed for each template with an appropriate primer.
Final constructs were verified by dideoxy double strand sequencing.
The sequence of the synthesized oligonucleotide was confirmed by the dideoxy method.
The method is known as the dideoxy chain termination method.
Positive clones were further characterized by southern analysis and dideoxy sequencing.
Sanger sequencing is also called dideoxy chain termination sequencing.
Resultant clones were orientated and sequence verified by fluorescent dideoxy sequencing.
The construct is sequenced by the dideoxy method to ensure correct assembly.
The compounds of the present invention are therefore dideoxy compounds.
DNA sequencing was performed by the dideoxy chain terminating method described by Sanger et al.
The nucleotide sequence of the isolated genes was confirmed by dideoxy sequencing.
The bromo deoxy intermediates were reduced to the dideoxy sugars by catalytic hydrogenation with palladium.
Four plasmid isolates were purified and sequenced using the dideoxy method.
Were confirmed by dideoxy sequencing.
The nucleotide sequence of the hybridized genome clone was determined by the dideoxy method.
Sequencing was performed using the dideoxy chain termination method.
Inserted cDNA base sequences of the phage DNAs were determined by the dideoxy method.
Nucleotide sequences were determined by a combination of dideoxy chain termination and chemical cleavage methods.
Plasmids are purified using standard techniques and sequenced using the dideoxy method.
Inserts were sequenced by the Sanger dideoxy chain termination method.
