Examples of 'dideoxy sequencing' in a sentence
Meaning of "dideoxy sequencing"
dideoxy sequencing: This phrase is a term used in molecular biology and genetics to describe a method of DNA sequencing. It involves the use of dideoxynucleotides to terminate DNA replication at specific bases, allowing the sequence to be determined
How to use "dideoxy sequencing" in a sentence
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dideoxy sequencing
The larger isolate was sequenced by dideoxy sequencing.
The dideoxy sequencing method was used.
All sequences were confirmed by dideoxy sequencing.
Samples suitable for dideoxy sequencing by capillary gel electrophoresis were prepared as follows.
The presence of the mutation is confirmed by dideoxy sequencing.
The sequence was determined by dideoxy sequencing of overlapping deletions generated by exonuclease III digestion.
Mutant sequences were confirmed by dideoxy sequencing.
The resulting dideoxy sequencing reactions were incubated at 48°C for 15 minutes.
The specificity of each product was confirmed by dideoxy sequencing.
Positive clones were subjected to dideoxy sequencing to confirm the mutagenesis.
Positive clones were further characterized by southern analysis and dideoxy sequencing.
All mutations were verified and confirmed by dideoxy sequencing using the Sequenase system ( USB ).
Resultant clones were orientated and sequence verified by fluorescent dideoxy sequencing.
All mutations are confirmed by dideoxy sequencing using the Sequenase system ( USB ).
The identity of the construct was confirmed by dideoxy sequencing.
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Were confirmed by dideoxy sequencing.
All deletion endpoints were characterized by restriction endonuclease mapping and dideoxy sequencing E.
The total dilution factor in the final dideoxy sequencing mix is at least 20-fold.
Positive clones were identified by plaque hybridization, restriction analysis and dideoxy sequencing.
All cloned sequences are sequenced using the Sanger dideoxy sequencing technique to verify the sequence.
One amplification was a LATE-PCR amplification, and the product was subjected directly to dideoxy sequencing.
The sequence of the selected RF was confirmed by dideoxy sequencing using a universal M13 primer.
All amplifications were LATE-PCR amplifications, and the product was subjected directly to dideoxy sequencing.
The sequence is obtained by the dideoxy sequencing method Sanger, F. et al.
The nucleotide sequence of the isolated genes was confirmed by dideoxy sequencing.
That product was submitted to the Brandeis University dideoxy sequencing facility for sequencing.
Amplification products will be directly sequenced using Sanger dideoxy sequencing.
The nucleotide sequences of the inserts were obtained by dideoxy sequencing using T3 and T7 primers.
The identities of all isoforms ( shown in Fig . 12 ) were confirmed by dideoxy sequencing.
The resultant plasmids are subjected to restriction endonuclease and dideoxy sequencing of double-stranded plasmid DNAs.
A similar procedure can be utilized for the analysis and dideoxy sequencing.
These were sequenced by Sanger dideoxy sequencing Sanger, F., et al.
The full length positive cDNA clone was sequenced using the dideoxy sequencing method.
Mutant sequences were confirmed by dideoxy sequencing in M13.
The clones were partially sequenced by Sanger dideoxy sequencing.
The sequence of the construct was confirmed by dideoxy sequencing of the DNA.
The fidelity of the PCR reactions was confirmed by dideoxy sequencing.
The ddF technique combines components of Sanger dideoxy sequencing with SSCP.
The cloned inserts were sequenced by double-stranded dideoxy sequencing.
The mutant sequence was confirmed by dideoxy sequencing in M13.
All PCR-derived fragments were verified by dideoxy sequencing.
The deletion mutants were sequenced using a M13 dideoxy sequencing kit ( Takara Shuzo ).
In another embodiment, said sequencing comprises dideoxy sequencing.
The sequence of the coding region was confirmed by dideoxy sequencing methods.
Small RNA library construction by bacterial cloning and dideoxy sequencing of inserts.
In another instance, said sequencing comprises dideoxy sequencing.
The linker sequence in pET - fabI was confirmed by dideoxy sequencing.
Both constructs obtained were verified by full-length dideoxy sequencing.
Both types are subcloned into sequencing vectors, and sequenced by the dideoxy sequencing method.
Finally, the DNA sequences are determined through dideoxy sequencing.
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Examples of using Dideoxy
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Several clones were sequenced by the dideoxy method
The dideoxy sequencing method was used
This invention relates to dideoxy nucleoside analogs
Examples of using Sequencing
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Appropriate sequencing of regulatory consolidation and liberalization
There was discussion regarding the appropriate sequencing of
The process and sequencing of reform were emphasized
