Examples of 'direct sequencing' in a sentence
Meaning of "direct sequencing"
a method of determining the precise order of nucleotides in a DNA or RNA molecule. It involves reading the sequence of bases directly without the need for cloning or amplification
How to use "direct sequencing" in a sentence
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direct sequencing
Such methods are preferably followed by direct sequencing.
Kits for carrying out direct sequencing of a polyribonucleotide.
All constructs were verified using direct sequencing.
Direct sequencing results.
The obtained amplimers were analyzed by direct sequencing.
Note that direct sequencing does not allow the identification of cysteine residues.
The resulting product was analyzed by direct sequencing.
Multiple direct sequencing attempts were made in both directions for all amplicons.
The orientation of cloning was confirmed by direct sequencing.
All mutations were confirmed by direct sequencing of the expression plasmids.
The amplified product is preferably detected by direct sequencing.
All vectors were confirmed by direct sequencing of the complete vector.
The resulting cloned plasmids were confirmed by direct sequencing.
This opens up the possibility to direct sequencing of nucleic acids without primer.
This method is also preferably followed by direct sequencing.
See also
Direct sequencing from patient samples should give a more accurate representation of true genetic diversity.
All the constructs were verified by direct sequencing.
Direct sequencing of the PCR products was also performed.
The amplified material was analyzed by direct sequencing.
Direct sequencing of the PCR products confirmed these results.
These findings were also confirmed by direct sequencing.
Direct sequencing of the PCR products can then be performed.
The mutations are confirmed by direct sequencing.
Techniques for the direct sequencing of DNA are well known in the art.
A positive clone is confirmed by direct sequencing.
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.
The sequence was confirmed by direct sequencing.
Was determined by direct sequencing of PCR products from the buffy coat DNA.
Preferably this method is followed by direct sequencing as well.
Direct sequencing of the genetic material ;.
Mutations in the genes were detected by method of pcr followed by direct sequencing.
The sequences can also be examined by direct sequencing of the encoded protein.
Methods were also developed for examining single base changes without direct sequencing.
PCR products were analyzed by direct sequencing as described above.
Newborns are analyzed for the presence mutated IgM exons by PCR and direct sequencing.
The cleaned PCR product was subjected to direct sequencing using the primers described above.
By direct sequencing tagged cDNA fragments, expression profiles could be generated.
The resulting mutations were identified by direct sequencing of the FokI domain.
Furthermore, direct sequencing of primer extension products may be employed.
More recently efforts have been made to develop direct sequencing methods.
In addition to the direct sequencing of the mutation, various methods may be used.
The genotyping results of every variant genotype above were confirmed by direct sequencing analyses.
The missing base was confirmed by direct sequencing of PCR amplified chromosomal DNA.
Additionally, the obtained product is a correct substrate for direct sequencing.
Moreover, the results are studied by direct sequencing and subsequent capillary electrophoresis.
To this end, SNPs between the parental genotypes were identified using a direct sequencing approach.
Prior to the present invention, direct sequencing protocols have exhibited a number of disadvantages.
SNPs can also be detected by direct sequencing.
Direct sequencing on a blotting membrane was unsuccessful, which was attributed to N-blockage.
In other embodiments the sequencing method includes a direct sequencing method without cloning.
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