Examples of 'ecori site' in a sentence
Meaning of "ecori site"
Ecori site: Refers to a DNA sequence recognized and cut by the restriction enzyme EcoRI. It is commonly used in molecular biology research to cleave DNA at specific sites
How to use "ecori site" in a sentence
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ecori site
Note that the EcoRI site is not reconstructed.
The resulting fragment is introduced into pCUP vía the EcoRI site.
The EcoRI site was reconstructed downstream of the acp gene.
The cDNA is inserted into the EcoRI site of the vector.
A single EcoRI site occurs in the center of the sequence.
Comprises an EcoRI site.
The results of the EcoRI site targeted integration are shown at the bottom.
This was ligated into the polylinker of pcDB at the EcoRI site.
The restoration of the EcoRI site is confirmed by digestion with EcoRI.
Start of the nucleotide sequence in the middle of the EcoRI site.
The oligonucleotide tag had an EcoRI site therein to facilitate later cloning procedures.
The TNFa cDNA was introduced between the SmaL and EcoRI site.
CPBII cDNA contained an EcoRI site in the inserted fragment.
Plasmids are prepared and analysed for the absence of the EcoRI site.
Each cDNA was subcloned into the EcoRI site of pBluescript SK.
See also
This fragment comprises only a single AccI site and a single EcoRI site.
Note that the EcoRI site was introduced in to this fragment by PCR cloning.
The adaptors are specific for either the EcoRI site or the MseI site.
Consequently, only the EcoRI site in the oligonucleotide tag was susceptible to EcoRI digestion.
It is known that this DNA only includes a single EcoRI site.
A plasmid containing a single EcoRI site was chosen and designated p4BARI.
The RNAi expression cassette can be inserted at the Pad site or EcoRI site.
A single-base mutation creating an EcoRI site is introduced to facilitate cloning experiments.
The first GGAATTC encodes a EcoRI site.
The sense primer contained an EcoRI site and the antisense, a BamHI site.
In addition, the integrase gene was deleted past EcoRI site.
This was cloned into the EcoRI site of lambda gtll ( a commercially available cloning vector ).
Using pCMV as backbone, minigenes were inserted in the EcoRI site of the polylinker.
The EcoRI site was introduced for sub-cloning purposes and lies downstream of the stop codons.
This ingeneous technique makes it possible, after various stages, to preserve the EcoRI site.
Following ligation, a unique EcoRI site results and those plasmids with intact MCSs can be selected.
However, each recombinant DNA molecule contains a cDNA segment at the EcoRI site.
Then the EcoRI site was destroyed by filling in, creating the vector pUC LTR RI -.
The cDNA with the adaptor was inserted in λZAP EXPRESS vector ( Stratagene ) at EcoRI site.
The sequence of the adaptor is, The EcoRI site facilitates the insertion of heterologous sequences.
This vector encodes a human Y1 gene downstream from the EcoRI site.
An additional EcoRI site was provided on the 5 ′ side of the fragment.
Subsequently, an End Rescue Site was ligated into the EcoRI site.
The downstream primer introduces an EcoRI site at the 5 ' end of the gene.
On the 5 ′ side the fragment was provided with an EcoRI site.
The 5 ' primer containing an EcoRI site and the 3 ' primer further includes a HindIII site.
A region ( upper bold case ) harbouring the EcoRI site.
EcoRI site integration efficiencies ( % ) were determined by ImageJ.
It was then transferred into a pUC19 vector into the EcoRI site.
This modified fragment was inserted into the EcoRI site of the expression vector pKRR810.
The composition of claim 20 wherein the restriction site is a EcoRI site.
In contrast, non-B type alleles lack the EcoRI site and have the BsmAI site.
In consequence, the 3 ' end of the gene is curtailed from the EcoRI site.
All three clones are cDNA inserts in the EcoRI site of pBluescript SK - ( Stratagene ).
The 3 ' primer also contains two stop codons prior to the EcoRI site.
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Only the relevant EcoRI sites are indicated
The plasmid was digested by restriction enzyme EcoRI
Underlined sequences indicate an EcoRI restriction site
