Examples of 'ecori fragment' in a sentence
Meaning of "ecori fragment"
ecori fragment: A segment of DNA that has been cut using the restriction enzyme EcoRI. This phrase is specific to the field of molecular biology and genetic engineering, where EcoRI is commonly used to cleave DNA at specific recognition sites for research purposes
How to use "ecori fragment" in a sentence
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ecori fragment
The cloning cassette described previously on a HindIII to EcoRI fragment.
An EcoRI fragment from a positive clone was subcloned into plasmid pZERO and partially sequenced.
From the library a genomic human PAC clone was isolated which hybridized to the EcoRI fragment.
The first EcoRI fragment of the cDNA contains a partial coding sequence within a complete amino terminus.
The NSs-protein gene is located on an EcoRI fragment.
The EcoRI fragment for both chicken and mouse is somewhat smaller than that for humans.
The two EcoRV fragments and an overlapping 5 kb EcoRI fragment were subcloned for further characterisation.
An EcoRI fragment containing around 4kB of promoter region was isolated.
The results revealed that the probe hybridised to a 4 kb EcoRI fragment in both cosmid clones.
The BglII to EcoRI fragment of Bbk was created in several steps.
A clone was obtained from the transformation, containing the desired EcoRI fragment in pLLK1 plasmid.
Nucleotide sequences of a 5 kb EcoRI fragment and a 2.6 kb EcoRI fragments were determined.
The EcoRI fragment from nucleotide 31 through nucleotide 409 was used to screen a human hippocampal cDNA library.
A transfer vector obtained from a pUC8 plasmid and comprising the EcoRI fragment 1 of the baculovirus cf.
Clones of the 4.25 kb EcoRI fragment were isolated by hybridization with the wild type fragment.
See also
The vector pBlue-E3-exIV was constructed similarly using a EcoRI fragment of the human apoE3 gene.
PBEl contained an EcoRI fragment of 8.5 kb derived from Pseudomonas cepasia.
In these PCR reactions, the 3 ' - untranslated region of the EcoRI fragment II insert was removed.
Example 8 A second EcoRI fragment from the positive clone described in Example 7, supra, was also subcloned.
A partial restriction map of the 9 Kb EcoRI fragment is shown in Figure 7.
The 4.0 kb EcoRI fragment was subjected to further analysis.
The fragment was isolated from gel and ligated to the 6kb Ad35 EcoRI fragment.
When probed with ant32, one EcoRI fragment from each clone hybridized.
The EcoRI fragment present in the di-alpha constructs is approximately 1450 bp.
A clone containing an approximately 2.3 kb EcoRI fragment was found to hybridize to the ART probe.
This 1.1-kb EcoRI fragment was cleaved with AhaIII, followed by addition of the XhoI linker.
The second probe was a 0.8 kb EcoRI fragment Van Ness et al.
The 12.5 kb EcoRI fragment was micro-injected into the male pronucleus of a single-cell mouse embryo.
The Asp to Ala 101 change is encoded on the NheI to EcoRI fragment.
The about 8-Kbp EcoRI fragment was recovered.
Probes III, IV and V all reside on the same 21 kb EcoRI fragment.
The resultant EcoRI fragment was subcloned in pBluescript SK ( + ).
Subcloning of 400-bp Sau3AI fragment in 8-Kbp EcoRI Fragment and Determination of Nucleotide Sequence Thereof.
For each Isq - mutant, the EcoRI fragment containing the Tn5 insertion was cloned into pBR322.
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Examples of using Ecori
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Only the relevant EcoRI sites are indicated
The plasmid was digested by restriction enzyme EcoRI
Underlined sequences indicate an EcoRI restriction site
Examples of using Fragment
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He has a fragment of sword embedded in his chest
There is a tiny missing fragment on the occipital
A small fragment impinging on her spinal cord
