Examples of 'eluted protein' in a sentence
Meaning of "eluted protein"
Eluted protein: Proteins that have been separated from a mixture using a technique called elution, where the proteins are washed out of a chromatography column with a solvent
How to use "eluted protein" in a sentence
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eluted protein
The eluted protein was identified as described above.
This ratio represents the % of the active protein in the eluted protein.
Eluted protein fractions were pooled for further purification.
After dialysis the eluted protein was evaporated to dryness.
Eluted protein was dialysed against PBS.
Following non-affinity chromatography, the eluted protein of interest is subject to HPTFF.
The eluted protein is finally refolded.
The PspA eluted from the column comprised at least 99 % of the eluted protein.
The eluted protein was then further analyzed by mass spectrometry.
Figure 5 shows SDS-PAGE analysis of the eluted protein.
The eluted protein can be stored without further treatment.
The continuous line represents the profile of eluted protein measured by O.D. 280.
The eluted protein fractions were tested in the bioactivity assay.
A Western dot blot was performed on the eluted protein using the monoclonal antibody 3F5.4G6.
The eluted protein was prepared for activation by removal of residual calcium.
See also
After purification, the concentration of protein in the eluted protein A pool was approximately 5-10 g / L.
The eluted protein had an apparent molecular weight comparable to yeast enolase.
Apparent molecular weights of eluted protein peaks were determined by interpolation using the calibration curve.
The eluted protein was quantified by UV absorbance and integration of peak areas.
The buffer containing the eluted protein was then transferred into a new multi-well plate.
The eluted protein was then dialyzed against Pubs.
The molecular weight of eluted protein was quantified with SEC as shown in FIG . 6.
Eluted protein samples were analysed by SDS PAGE.
The identity of the eluted protein was confirmed by immunoreactivity using anti human IgG FIGS.
Eluted protein samples were analyzed by SDS PAGE.
A 15mL fraction containing the eluted protein as judged by SDS-polyacrylamide gel electrophoresis was collected.
The eluted protein is quantified by UV absorbance and integration of peak areas.
The homogeneity of the eluted protein was analyzed by gel electrophoresis ( SDS-PAGE ).
The Eluted protein is dialyzed into PBS before injection to rabbits.
The concentration of the eluted protein was determined by A280 and amino acid analysis.
The eluted protein was then dialyzed against PBS.
Monitor the eluted protein by absorbance at 280 nm.
Eluted protein was dialyzed ON to PBS.
Alternatively, the eluted protein can be dialyzed against 50 % acetic acid in water.
Eluted protein fractions from step 2 were pooled for further purification.
Subjecting the eluted protein to gel filtration or to ion exchange chromatography ; and.
Eluted protein was pooled, concentrated and further purified by size exclusion chromatography.
Purity of the eluted protein was checked by loading fractions on a SDS gel . Results.
The eluted protein was then dialysed overnight against 1 l of phosphate buffered saline prior to use.
Purity of the eluted protein was checked under reducing and non-reducing conditions by SDS-PAGE.
Eluted protein was separated by SDS-PAGE and analyzed by autoradiography.
The pooled eluted protein was analyzed using SDS PAGE with silver staining, confirming expression.
Eluted protein fractions from the step using 100 % buffer B were pooled for further purification.
Purity of the eluted protein was assessed using SDS-PAGE and size-exclusion chromatography ( SEC ).
The eluted protein was greater than 95 % pure after elution as judged by SDS-polyacrylamide gel electrophoresis.
Purity of the eluted protein was assessed by SDS-PAGE and by two-dimensional IEF / SDS-PAGE.
The eluted protein was resolved by SDS-PAGE and stained with silver staining.
Detection of the eluted protein was performed by measuring the absorbance at 220 nm and 280 nm.
The eluted protein was mixed in semi-synthetic diet.
The eluted protein could be directly analyzed by SDS-PAGE.
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