Examples of 'elution step' in a sentence
Meaning of "elution step"
elution step: In the context of chemistry or biology, this phrase refers to the process of separating or extracting a substance from a matrix or mixture through the use of a solvent or solution
How to use "elution step" in a sentence
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elution step
The elution step is repeated a second time.
During the elution step.
Then an elution step is effected by injecting elution buffer.
Five column volumes of the eluted fraction was collected in each elution step.
The elution step can be repeated and supernatants pooled.
Six column volumes of the eluted fraction was collected in each elution step.
A final elution step leaves only purified tripartite complexes.
Five column volumes of the eluted fractions was collected in each elution step.
The elution step was an incubation for ten minutes with mixing.
The polyethylene glycol and glycine combination serves to stabilize the protein during the elution step.
To improve the elution step the elution buffer may comprise salt.
SP is the duration setpoint of the elution step.
The elution step may be repeated a second time and combined with the first elution volume.
In some embodiments, the elution step is repeated.
The elution step by pumping is effected at substantially constant pressure ;.
See also
In this case, there is no need of an elution step.
Thus, the elution step can be performed as a dynamic or batch procedure.
About 2 column volumes of eluent are necessary in this elution step.
This elution step was performed three times . Results.
Just as before, the flow direction for the elution step was reversed.
The elution step was repeated five more times and the supernatant fractions analysed by SDS-PAGE.
According to the invention, a neutralization buffer is added after the elution step.
The recorded elution profile from this first elution step is indicated at Figure 1.
In one embodiment of the disclosure, the method comprises more than one elution step.
The elution step was repeated with an additional 60 µL water.
Impurities are washed off the ion exchange chromatography column before the elution step ( f ).
The elution step was repeated 4 times.
About 2 column volumes of Elution Buffer were necessary in this elution step.
This elution step is repeated once to get a total elution volume of 60 µl.
About 2 column volumes of Elution Buffer were used in this elution step.
The elution step was repeated with a second volume of 50 µl RNase-free water.
Lane 12 corresponds to the fraction obtained after the first elution step.
The elution step was performed in a separate elution tube that is heated to 70°C.
Preferably, the pH of the eluant during the elution step is less than about 6.
The elution step was repeated with 15 µl of 1X elution buffer and the eluates were pooled.
Impurities are washed off the hydrophobic interaction column before the elution step ( d ) ; and.
After completion of the elution step ( B ), the displacement step is carried out.
Preferably, around 2 mM EGTA is added for the elution step.
The elution step is again performed and the samples thus obtained are rapidly frozen at -80 ° C.
After collection of the 300 microliter, the elution step was repeated.
The elution step was repeated again by adding 200 µl of CBST and incubating for 5 minutes.
Five ml fractions from each washing and elution step ( 180 fractions in total ) were collected.
The elution step is repeated with a further 250µl of Elution buffer and the eluates combined.
The second pool consisted of fractions 85 to 125 collected during the pH gradient elution step.
Most preferably, the elution step of the present invention comprises 1.2 bed volumes.
Additionally, urea ( 6M ) may be added during the elution step.
The method of claim 3, wherein said elution step comprises using an elution volume of 1.2 bed-volumes.
Complete the final elution step in 100 µl AE buffer.
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Column elution method with recirculating pump
Results obtained after elution and neutralization
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