Examples of 'forward primer' in a sentence
Meaning of "forward primer"
In the context of molecular biology or genetics, a 'forward primer' is a short DNA sequence that is designed to bind to a specific target region and initiate DNA replication
How to use "forward primer" in a sentence
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Advanced
forward primer
Usually the forward primer is labelled.
If no primer fulfils the conditions try another forward primer design.
Each forward primer was designed to amplify preferentially a specific codon.
Each pair of primers comprises a forward primer and a reverse primer.
The forward primer is added and anneals to the circularized probe.
The terbium probe is complementary to the extension product of the forward primer.
The positions of the forward primer and reverse primer are indicated.
The capture probes capable of hybridizing to extension products of the forward primer were selected.
The universal forward primer binding site is complementary to the universal forward primer.
This can be achieved by using different ratio of forward primer and reverse primer.
One forward primer suitable for use with the assays described herein is a NuAf primer.
The universal primer pair includes a forward primer and optionally a reverse primer.
The forward primer used by Urabe et al.
The mutation sites should be close to the forward primer or the reverse primer.
The Forward primer was decided ex novo.
See also
It is understood that the methods comprise at least one forward primer and a reverse primer.
The forward primer incorporates a SalI restriction site and a RBS.
The first PCR amplification was completed using an outer forward primer and a reverse primer.
The forward primer anneals to a region common to multiple Pgp clones.
Half of the RT reaction was subjected to PCR using a radioactively labeled forward primer.
The locations of the reference forward primer and reverse primer are indicated by shaded boxes.
Forward primer to amplify the protein coding sequence of the barley gene for MMT.
The PCR was performed using each forward primer in conjunction with a relevant reverse primer.
The forward primer incorporates EcoRI and MluI restriction sites and a RBS.
It is preferred that the forward primer is SEQ ID NO.
The forward primer incorporated a SalI restriction site and a RBS.
In some embodiments, the forward primer is a NuAf primer.
The forward primer incorporated an Hpai restriction site and a RBS.
In such cases, the template oligonucleotide serves as a forward primer.
In some embodiments both the forward primer A and the reverse primer have a counter sequence.
More preferably, it may be localized in the proximity of the forward primer.
The forward primer incorporates a Sall restriction site and a RBS.
Furthermore, for purification purposes, forward primer included a protease cleavage site.
The forward primer incorporated a Sall restriction site and a RBS.
Likewise, the second primer can be designed as a forward primer or a reverse primer.
F stands for forward primer and R stand for reverse primer.
Likewise, the second primer may be designed as a forward primer or a reverse primer.
The forward primer incorporated EcoRI and Mlul restriction sites and a RBS.
Then, thermal cycling is continued with effectively only the forward primer.
The sequence for the forward primer for the fluorescent Fragment A probe was as follows.
Typically, the spacer region contains a binding site for at least one primer, the forward primer.
Nucleic acid Forward primer to detect alternative splicing of the gene encoding barley MMT.
The selected mutant template is amplified specifically using a real-time fluorescent forward primer and reverse primer.
F as forward primer and the following reverse primer,.
The method of claim 3, wherein the forward primer contains one or more ribonucleotides.
The forward primer may comprise one, two or even more LNAs.
Preferably, a pair of primers such as a forward primer and a reverse primer, and a probe are applied.
A forward primer may, for example, be used to amplify fragments between two reverse primers.
The blocker repeats, anchor sequence and forward primer are all included in a single oligonucleotide.
The forward primer hybridizes with the target sequence 5 ′ with respect to the reverse primer.
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Examples of using Forward
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I shall look forward to seeing you again
I am just gonna bend your head forward
I look forward to reading about your successes
Examples of using Primer
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The primer includes the following modules
It was mostly gray primer and fender fill
A primer is not necessary for small repairs
