Examples of 'forward primers' in a sentence
Meaning of "forward primers"
forward primers: In genetics and molecular biology, forward primers are short, single-stranded DNA sequences used to initiate the polymerase chain reaction (PCR) to amplify a specific region of DNA
How to use "forward primers" in a sentence
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forward primers
The designation of reverse and forward primers may be switched.
Forward primers were pooled together in ratios which reflected their degree of degeneracy.
Similarly disclosed are methods comprising at least three forward primers.
A population of forward primers may contain duplicates of any one sequence.
The mutation sites are indicated by bold letters in the mutagenic forward primers.
A set of forward primers in combination with barcodes is used for linking and sequencing.
A change in temperature can also selectively induce a conformational change in the unextended forward primers.
The forward primers illustrated in Fig.
The hairpin oligonucleotides are used as forward primers during PCR.
Suitable forward primers are by way of example,.
In a preferred embodiment, first nanoparticles are conjugated to forward primers.
The forward primers were included in the SMART-Kit.
In this embodiment, the test sequences are complementary to the forward primers.
Forward primers contained an AscI site, followed by a rabbit splice site.
The design of the gene-specific forward primers was based on obtained sequences.
See also
Forward primers were designed to include three nucleotides 5 ′ of the start Met site.
The reverse primers when paired with forward primers will amplify each ORF, respectively.
The forward primers and the probe used in quantification of PRRSV is the ORF7 region.
The subsequent amplification was performed with the same forward primers and the VK2BACK primer.
In certain cases, forward primers comprising one or more cleavable bonds are used.
Fig . 1 schematically illustrates a population of forward primers.
In some embodiments, the forward primers are selected from a NuAf primer and a TATA primer.
These oligonucleotides described in ( a ) to ( d ) above may be referred to as forward primers.
Example 3 This example describes alternative forward primers and detectable oligonucleotides for use in the methods.
The forward primers ( murine IgG leader ) are provided below.
Primers sequences, where F denotes forward primers and R denotes reverse primers,.
The forward primers are also referred to as upstream, sense or 5 ' primers.
Note that regardless of which gene is amplified, the forward primers remain constant ( b ).
The four forward primers were,.
Forward primers were synthesized with a 5 ' phosphate, whereas reverse primers were not.
The sequence and names of the 28 forward primers are listed in Table 1.
Forward primers and lengths of pCS1 and its variants are listed in Table 13.
Primers with names starting with " Clon " are the forward primers.
Another sub-population of forward primers 10b are not hybridized to the target polynucleotide.
Forward primers used in combination with the AG278 K reverse primer.
Selectively disabling, after step ( d ) any forward primers that are not extended ; and.
PROPERTIES, 5 ' forward primers for PCR amplification of murine VH domains.
Such a population of forward primers is schematically illustrated in Fig . 1.
Some of the forward primers are longer to incorporate a G or C at the 3 ' end.
In other embodiments, both forward primers 10 and reverse primers 16 may be selectively disabled.
As shown in Figure 10 ( j ), the forward primers hybridize to the RPE portion of the RPA.
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I shall look forward to seeing you again
I am just gonna bend your head forward
I look forward to reading about your successes
Examples of using Primers
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For final sanding of primers before coating
Alkyd primers utilise corrosion protection pigments
Hybridisation of the primers with the template
