Examples of 'fucosylation' in a sentence
Meaning of "fucosylation"
fucosylation (noun) - a biochemical process of adding fucose sugars to molecules, often proteins, which plays a role in various cellular functions
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- glycosylation using fucose moieties
How to use "fucosylation" in a sentence
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fucosylation
Fucosylation of proteins is believed to play a role in mammalian development.
Aleuria aurantia differentiates on the basis of core fucosylation.
Fucosylation is the process of adding fucose sugar units to a molecule.
Methods of inhibition of protein fucosylation in vivo using fucose analogs.
Fucosylation relates to the adding of fucose sugar units to a molecule.
The invention also relates to conditional control of protein fucosylation.
Fucosylation is the process of transferring a fucose residue to various proteins and oligosaccharides.
Such cells are advantageously cells having a low or no fucosylation capacity.
The selectins require sialylation and fucosylation of most carbohydrate ligands for recognition.
Umana notes that such bisected molecules result in less fucosylation.
The collected antibodies were evaluated for fucosylation levels by dot blot as follows.
Feeding such cells with fucose will theoretically rescue glycoprotein fucosylation.
Such a resting period can allow restoration of fucosylation of proteins involved in essential functions.
The glycans also usually have high levels of core fucosylation.
Fucosylation of antibodies and other proteins can be modulated using the fucose analogs described herein.
See also
Hematopoietic stem cells treated by in vitro fucosylation and methods of use.
The present invention also provides specially engineered cell lines which achieve a respective low overall fucosylation.
Comprises a variant Fc region and has reduced fucosylation or is afucosylated.
Fucosylation variants may have improved ADCC function.
These assays identified candidate compounds for inhibition of core antibody fucosylation in mammals.
Fucosylation and Cancer.
The remaining collected antibodies also exhibited a reduction in core fucosylation of about one quarter.
Therefore, loss of fucosylation has significant biological consequences.
Also described are specially engineered cell lines which achieve a respective low overall fucosylation.
Also contemplated are antibody molecules with absent or reduced fucosylation that exhibit improved ADCC activity.
Methods and compositions for making antibodies and antibody derivatives with reduced core fucosylation.
An example of the increase in fucosylation is shown in figure 2.
The Lentil lectin was used to fractionate recTSH according to its fucosylation state.
For example, fucosylation rates can be different during fermentation.
The Fc region may have reduced fucosylation.
Has reduced fucosylation or is afucosylated ; or.
This allowed us to assign epitope III as being under the control of core fucosylation.
In a specific embodiment, the percent fucosylation is a mole percent of fucose to glycoprotein.
The efficiency of the ADCC mechanism is considerably dependent on the level of antibody fucosylation.
In a specific embodiment, the percent fucosylation is a mole percent of fucose to glycan.
The % fucosylation compared to untreated is shown in the graph on the right.
In a preferred embodiment, an antibody may be produced with varying amounts of fucosylation.
Purification of the fucosylation product 3-fucosyllactose produced as described above was purified in several steps.
Currently, however, there are no approved therapeutic approaches targeting protein fucosylation levels.
After correcting for loading level, the % fucosylation is shown in the graph on the right.
The term antibody includes antibodies that are non-fucosylated or have reduced core fucosylation.
In one embodiment the modified oligosaccharides have reduced fucosylation as compared to non-modified oligosaccharides.
Therefore, feeding cells that have such a knockout with fucose will not rescue glycoprotein fucosylation.
In one embodiment the modified oligosaccharides have reduced fucosylation as compared to not-modified oligosaccharides.
Fucosylation reaction conditions and substrates . The fucosyltransferases are typical glycosylases and are relatively hardy enzymes.
Binding is rather weak indicating an uncomplete but effective fucosylation of the N-Glycans.
Fucosyltransferases . Fucosylation arises from the action of a fucosyltransferase.
Therefore, fucose biosynthetic pathway and cellular protein fucosylation still remains active.
In one embodiment, N-glycan core fucosylation of the glycoprotein is inhibited by a fucosylation inhibitor.
The fucose / mannose peak área ratios were used to determine the fucosylation state of the antibodies.
