Examples of 'helper phage' in a sentence
Meaning of "helper phage"
A helper phage is a type of bacteriophage that assists in the replication or transfer of another phage, often used in molecular biology techniques such as phage display or recombinant DNA technology
How to use "helper phage" in a sentence
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helper phage
No binding was detected with helper phage alone.
Helper phage was used as a control.
These phage genes can be provided by a helper phage.
Helper phage may be modified in order to render them cleavable by a protease.
Infectious phage particles can be obtained vía helper phage.
The phage was rescued by helper phage for next round of panning.
This strain provides gIII protein in trans to the helper phage.
The use of helper phage superinfection lead to expression of two form of cpIII.
The phage is then isolated and the phagemid is excised with a helper phage.
This means that helper phage need not be added to make phage.
Monovalent display can be accomplished with the use of phagemid and helper phage.
The use of helper phage superinfection lead to expression of two forms of cpIII.
It is known that bacteria grow slower when infected with the helper phage or virus.
An alternative to the helper phage is the use of a plasmid encoding the missing functions.
Phage library was then made by infection the mutational library with the helper phage.
See also
Helper phage did not give a signal in the ELISA.
Single stranded DNA template was purified from phage following infection with helper phage.
Infection with a helper phage produces more template, making sequencing possible.
Alternatively, phage display without the use of helper phage may be utilized.
The use of a helper phage can be eliminated by using ' bacterial packaging cell line ' technology.
The capsid protein may be absent, defective or conditionally defective in the helper phage.
Preferably, however, said host is provided with a helper phage according to the invention.
Expression of the bacteriophage in bacteria can, in some embodiments, require use of a helper phage.
The use of helper phage superinfection allowed for the expression of two forms of cp3.
If a phagemid vector is used, then this step requires superinfection with helper phage.
Preferably, said helper phage does not comprise nucleic acid encoding said fusión protein.
In one embodiment of the invention, said infection step includes the use of a helper phage.
However, this led to the incorporation of more helper phage genomes rather than phagemid genomes.
The shorter g3p part would not prevent the generation of a viable and useful helper phage.
In one aspect, the invention comprises a mutant helper phage for enhancing phage display of proteins.
Neither helper phage alone nor intact E. coli labelled with antigen.
For example, if the cloning vector is a cosmid, the helper phage will necessarily be a X phage.
The D3 helper phage genome is identical to the CT helper phage genome.
The vector is electroporated in E. coli and the E. coli is infected with helper phage.
The cultures are infected with helper phage VCSM13 to generate more packaged phagemids.
Bluescript ™ plasmids were rescued from plaque purified clones by excision with M13 helper phage.
Colonies were isolated and grown with helper phage to obtain single-stranded DNA for sequencing.
A phagemid clone was selected to produce viral particles after infection with the helper phage M13KO7.
B - Functionality of pVII donated by helper phage in phagemid pIII display,.
VCSM13 helper phage were added for infection as described above.
The infectious gene Ill-protein is provided by the helper phage VCSM13.
The cultures with helper phage were rescued overnight by incubation at 37°C.
The eluted output was used to infect TG1 F+ bacteria, rescued with helper phage.
Helper phage bring into the E. coli wild-type pIII that competes with the scFv-pIII fusión.
Phagemids were released vía co-infection with helper phage and recircularized to generate Bluescript SK - ( Stratagene ).
Positive phage clones were converted into plasmids by co-infection with ExAssist Helper phage ( Stratagene, CA ).
Using M13VCS helper phage infection, the VHH library was expressed on phages.
The transformed bacteria were transfected with VCSM13 helper phage Stratagene, Cat.
A helper phage obtainable by a method according to any of the claims 7-13.
In some embodiments, no helper phage is required see, e.g., Chasteen et al.
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