Examples of 'helper plasmid' in a sentence
Meaning of "helper plasmid"
helper plasmid: A type of plasmid used in molecular biology to assist in the replication of recombinant DNA
How to use "helper plasmid" in a sentence
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helper plasmid
The helper plasmid may be added before or simultaneously with the integrative plasmid.
The adenovirus genes may be placed into the helper plasmid in any positional order.
The helper plasmid is not packaged in significant amounts due to a lack of ITR sequences.
The retroviral vector can be produced in a packaging cell line containing a helper plasmid or plasmids.
Accordingly, the helper plasmid can neither replicate nor package itself.
The intermediate vector is moved into the Agrobacterium by a helper plasmid or by electroporation.
By means of a helper plasmid the intermediary vector can be transferred to Agrobacterium tumefaciens ( conjugation ).
The experiments were performed in the presence and absence of the TMPRSS helper plasmid.
Where the target strain is RecA -, a helper plasmid expressing recA may be necessary.
Super-binary transformation vectors are used in conjunction with a helper plasmid.
A particular embodiment of such a replicating helper plasmid according to the invention is shown in FIGURE 1.
The intermediate vector can be transferred to Agrobacterium tumefaciens using a helper plasmid ( conjugation ).
Cultures with chaperon helper plasmid pGF134 were also more repeatable and stable.
The intermediate vector can be transferred into Agrobacterium tumefaciens by means of a helper plasmid ( conjugation ).
The helper plasmid RSF-Red-TER was used as a carrier of λ phage Red genes.
See also
The resistance genes are then eliminated by using a helper plasmid encoding the FLP recombinase.
Restriction map of fragment of Ti-plasmid pTiB6 used for the construction of a disarmed helper plasmid.
Tumefaciens by means of a helper plasmid ( conjugation ).
PTOK253 is a plasmid which can constitute a binary vector by combination with a helper plasmid.
An example of a non-replicating AAV helper plasmid is provided in FIGURE 3.
A corresponding helper plasmid can be constructed as described for example by Markowitz et al ., J. Virol.
The intermediate vector can be transferred into Apobacterium tumefaciens by means of a helper plasmid ( conjugation ).
FIG . 8, Characterization of helper plasmid pVP2Ncm-gfp with alternative codon usage.
The intermediate vector may be transferred into A. tumefaciens by means of a helper plasmid ( conjugation ).
An Agrobacterium strain according to claim 4, wherein said helper plasmid does not contain T-DNA borders.
FIG . 2F is a schematic showing the AAV components of the rep-AAP helper plasmid.
The pW1909 helper construct and the pAAV / Ad helper plasmid were used as controls.
In case of AAV promoter p40 dependent transcription a co-transfection with an adenoviral helper plasmid was performed.
An Agrobacterium strain according to claim 3, wherein said plasmid is a helper plasmid harbouring vir - functions.
CRL 1658 are for example firstly transfected with the gag / pol helper plasmid.
Fig . 2 is a drawing which shows construction of helper plasmid RSF-Red-TER.
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