Examples of 'hepes' in a sentence
Meaning of "hepes"
Hepes is a term commonly used in biochemistry and research labs, representing a buffer solution with high buffering capacity at a specific pH range
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- 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, a zwitterionic buffering agent.
How to use "hepes" in a sentence
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hepes
Prepare hepes saline and probenecid solutions fresh for each assay.
Thus in the equine application the sheath fluid is selected which contains the hepes buffer.
It is possible that Hepes be a mozarabic toponym.
Hepes buffer as a substitute for PBS shows no benefit.
Dissociation was initiated by superfusion with Hepes buffered saline.
Other buffering systems such as hepes or glycine-NaOH and potassium phosphate buffers can be used.
Fractions containing virus are dialyzed against Hepes buffered saline.
In the control group, Hepes buffer described above was charged.
For sequence analysis the fractions were collected without Hepes buffer.
The less temperature sensitive Hepes buffer was chosen for the assay.
An alternative solution is Hepes.
Coverslips were repeatedly washed with Hepes Tyrode to completely remove adhesive platelets.
Preferable, the buffer of the present invention is Hepes.
Radioligand and membranes are diluted in Hepes buffer + 1 mg / ml bacitracin.
Optionally, the formulation further comprises buffer, such as Tris buffer or Hepes.
See also
After perfusion the coverslips were gently washed with Hepes Tyrode, taken from the chamber.
Antigen uptake experiments DC were resuspended in medium buffered with 25mM Hepes.
Also an aqueous solution of PEG in 10 mM Hepes buffer was prepared.
The eluting solution is DMEM, low glucose with glutamine and Hepes.
SC7 bolasomes were prepared in 5 mM Hepes buffer at a concentration of 10 mM.
The specific reagent is the same as that of example 2 with the Hepes buffer.
When employing Hepes or histidine, the pH may optionally be adjusted to 6.5 to 7.
The method of claim 8, wherein the formulation buffer further comprises Hepes buffer.
The obtained cells were suspended in a Ca-Mg Hepes culture medium to a concentration of 1 x 106 cells / ml.
Subsequently, the column was washed with 10 volumes of the named Hepes buffer.
Both proteins were diluted in the Hepes buffer, to give a final well volume of 100µl.
The concentration ranges of compounds used for such a determination are, Hepes or alternative chloride.
Both proteins were diluted in the Hepes buffer as above ; final well volume was 100µl.
The solution was removed and cells were washed twice with cold Hepes buffered saline ( HBS ).
Hundred µl of Hepes buffer is brought into all ( N+1 ) *8 wells with an eight channel pipette.
Other suitable buffers may include sodium acetate, Hepes buffer, etc.
The final sample was diluted in Hepes buffer with EDTA and stored at -20°C.
In one embodiment, activated / pre-activated FXIII is measured in the presence of Hepes buffer.
The volume was taken to 10.5 mL with the above Hepes buffer to yield a cloudy solution.
Labelled cells were washed three times with DMEM, supplemented with 10 mM Hepes.
The immobilisation procedure was performed with a continuous flow of Hepes buffer ( 5 µl / min ) at a temperature of 25°C.
Other buffers may comprise acetate, carbonate, bicarbonate, phosphate, citrate, tris or hepes.
The preparation was then dialysed against Hepes buffer and stored and -80°C.
The washing reagent is conveniently an aqueous buffer, e.g. Hepes.
The less temperature sensitive Hepes buffer was chosen for the assay . ( FIG . 2C ) Impact of pH.
A method according to Claim 11 wherein the buffer is Hepes buffer.
The reagent of claim 24, wherein the buffer system contains Hepes.
Load cell plate and drug plate into FLIPR ; run experiment . Hepes saline - 1L.
The elutriation was performed in Earle 's balanced salt solution pH 7.4 buffered with 10 mM Hepes.
A method according to Claim 17 wherein step ( b " ) comprises adding Hepes buffer.
The umbelliferone and rhodamine are added as 600 µM stock solutions in 50 µL of Hepes buffer.
The internal solution also contains 10 mM EGTA and 5 mM Hepes.
The collected fractions were immediately neutralized to pH 7.0 with 1 M Hepes buffer.
The recombinant HA ectodomain were eluted with 500 mM imidazole in 20 mM Hepes buffer.
B2-GPI solution, The solution was prepared by diluting purified human B2-GPI with Hepes buffer.
