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hindiii restriction
The chromosomal DNAs were digested by the HindIII restriction enzyme.
NheI and HindIII restriction digests were used to create compatible ends.
The site lies between the HinII and HindIII restriction sites.
PacI and HindIII restriction sites flank the scMspA nanopore octamer sequence.
The entire MspA porin sequence is flanked by PacI and HindIII restriction sites.
All 12 colonies contained the HindIII restriction site at the position of the SmaL site.
Plasmid DNA is isolated and analysed by EcoRI and HindIII restriction digests.
The HindIII restriction map of the recombinant IBRV ( NG ) dltkdlgIII of the present invention is also shown.
At the ends of these primers HindIII restriction enzyme sites were located.
The isolated genomic DNA was digested with either EcoRI or HindIII restriction enzyme.
This cDNA was provided with HindIII restriction sites and cloned in pUC18.
The 5 ' end of primer B also contains nine nucleotides which form a HindIII restriction site.
The HindIII restriction map of IBRV ( NG ) dltk virus DNA is also shown in Figure 1.
The DNA was cut with NsiI and HindIII restriction enzymes.
Clones were selected, DNA isolated and analysed by digestion with BamHI and HindIII restriction enzymes.
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Primer 1 contains an added HindIII restriction site at its 5 ' end.
The PCR product was cloned into pQe30 expression vector using BamHI and HindIII restriction sites.
Primers contained the NdeI and HindIII restriction sites, respectively.
Note that pSBS3400 is a plasmid that contains the D9 scFv-KDEL fragment with a HindIII restriction site.
In addition this fragment contains a XhoI and a HindIII restriction site at its 3 ' end.
The nucleotide change at position 1473 eliminates a HindIII restriction site.
All 19 showed the predicted Sma I and HindIII restriction fragments.
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The HindIII site is designated by a vertical line
Cleavage site of the restriction enzyme HindIII
The restriction sites HindIII and NcoI are noted in italics
