Examples of 'histidine tag' in a sentence
Meaning of "histidine tag"
histidine tag: a specific amino acid tag used in biochemistry and molecular biology for protein purification
How to use "histidine tag" in a sentence
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histidine tag
The protein was expressed with histidine tag.
Preferably this histidine tag consists of five histidine residues.
The protein was expressed without histidine tag.
The histidine tag can simplify processing and purification.
The initiating methionine precedes the histidine tag.
Histidine tag purification systems are known to persons of ordinary skill in the art.
Iodide protein was further functionalized vÃa incorporation of a histidine tag.
N terminal histidine tag.
The protein can further be prepared without a histidine tag.
Proteins comprising a histidine tag can be purified using immobilized metal affinity chromatography.
Hybridomas secreting antibodies directed against the histidine tag were discarded.
A histidine tag has been incorporated to allow rapid purification of scFv by nickel chelate chromatography.
The capture antigen for this experiment was an equivalent truncated FimH without a histidine tag.
The histidine tag is not immunogenic and does not interfere with antibody detection.
The subdomain is underlined and the amino acids encoding the histidine tag are in italics.
See also
The gene encoded an additional histidine tag sequence to simplify purification of the recombinant protein.
Fab His was the Fab fused to a histidine tag.
The Kit kinase was engineered to include a Histidine tag for purification using metal affinity chromatography.
I-metabolite was further functionalized vÃa incorporation of a histidine tag.
The Tat derivatives may be expressed with a histidine tag useful for isolation of the polypeptide.
Iodide X-Gal was further functionalized vÃa incorporation of a histidine tag.
In certain embodiments, the histidine tag is a hexahistidine peptide.
In one embodiment, the fusión protein comprises a histidine tag.
In embodiments, the histidine tag comprises at least six histidines in sequence.
In some embodiments, the antibody comprises a histidine tag for purification.
If a histidine tag is used, elution is most conveniently achieved by way of a imidazole gradient.
The collagen is cleaved from the 6 histidine tag with enterokinase.
If desired, the histidine tag can be selectively cleaved with an appropriate enzyme.
Alkaline phosphatase is at the end of the antibody fragment, followed by a histidine tag.
Particularly, the use of the histidine tag having a smaller molecular weight is desirable.
The construct of claim 1 further comprising a third nucleotide sequence encoding a histidine tag.
In certain embodiments, the purification tag is a histidine tag or a substantially identical variant thereof.
The 6 histidine tag allows also to purify the enzymes using nickel affinity chromatography.
In this purification method, therefore, the histidine tag is cut off after purification.
The method according to any preceding claim, wherein the disulphide isomerase comprises a histidine tag.
The recombinant protein is purified with a histidine tag affinity column, according to the manufacturer 's protocol.
Thus, plasmid DNAs capable of expressing an antigenic peptide fused with a histidine tag were prepared.
A histidine tag was fused to the C-terminus.
Incorporation of histidine . I-metabolite was further functionalized vÃa incorporation of a histidine tag.
Preferably the histidine tag is found at the C-terminal of the polypeptide.
In a preferred embodiment, a KSP fragment comprises a myc epitope and a histidine tag.
A six histidine tag was added to the C-termini of the light chains.
Moreover, it was confirmed that a histidine tag had been added to the C-terminus.
The ECD-PD insert was fused in frame with a N-terminal 6 x histidine tag.
In some embodiments, the histidine tag contains the sequence HHHHHH.
This resulted in the addition of an inframe C-terminal V5 epitope and 6x histidine tag.
In some embodiments, the histidine tag comprises the sequence IIIIIIIIIIII.
As can be seen in Figure 2, versions B and E of pMORPH25 effectively destroyed the histidine tag.
In some embodiments, the histidine tag comprises the sequence HHHHHH.
In another embodiment, the epitope tag is c-myc, hemagglutinin, or a histidine tag.
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Carnosine is made from alanine and histidine
A histidine residue was identified as potential heme ligand
Examples of using Tag
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Add that price tag to your hunt
This tag information is already stored in memory
He was being fitted with a tag when he went out of control
