Examples of 'histidine residues' in a sentence
Meaning of "histidine residues"
Histidine residues refer to specific amino acid components in proteins that contain the amino acid histidine. These residues play key roles in various biological processes and interactions
How to use "histidine residues" in a sentence
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histidine residues
Histidine residues typically are involved in such interactions.
Preferred derivatization sites are at histidine residues.
The histidine residues enabled affinity purification of the protein.
This particularly applies to two or three histidine residues.
The total number of histidine residues may vary in the tag.
Preferably this histidine tag consists of five histidine residues.
Two histidine residues from each subunit coordinate the heme.
It is surprising that high selectivity to two histidine residues may be obtained.
Shh histidine residues are shown as sticks with nitrogen atoms shaded dark.
The polyhistidine tag is preferably a string of at least six histidine residues.
Histidine residues can be incorporated to allow immobilized metal affinity chromatography purification.
Urethane linkages are capable of interacting with lysine and histidine residues.
The histidine residues allow the purification of the recombinant protein by affinity chromatography.
All the peptides of the invention comprise two consecutive histidine residues.
Conserved cystein and histidine residues are colored with red and blue colors respectively.
See also
Examples of such affinity peptides are peptides containing at least two histidine residues.
The histidine residues are found at the active site of ribonuclease and several other enzymes.
A particularly useful alteration is the insertion of two or more neighboring histidine residues.
None of the other histidine residues when mutated to alanine show a complete loss of activity.
The four copper atoms are coordinated by seven histidine residues and bridged by a sulfur atom.
Histidine residues introduced through mutagenesis and corresponding nucleic acid residues are shown in bold.
This allows purification of proteins containing a tag consisting of six histidine residues at either terminus.
Two conserved histidine residues and a glutamate immobilise the zinc ion at the active site.
One example of insertions includes a short stretch of histidine residues e . g.
In one embodiment the number of histidine residues is of from 4 residues up to 8 residues.
Polyhistidine tags used in the present invention contain up to 12 histidine residues.
The construction also encoded six additional histidine residues at the amino terminus of the protease.
The histidine residues enabled affinity purification of the protein ( vide infra ).
In this method, the polymer is conjugated to histidine residues in a polyhistidine tag.
Most of such pH-dependently changing protein-protein interactions are associated with histidine residues.
With respect to the protein, the histidine residues are positioned in a polyhistidine tag.
Here, two copper atoms are each coordinated with three histidine residues.
Increasing the number of histidine residues further to 7 or 8 does not increase the capture efficiency.
Typically, the heptads will not comprise any proline or histidine residues.
Thirty-two of sixty-seven histidine residues of cytochrome oxidase are accessible to modification by diethyl pyrocarbonate.
In preferred embodiments, conservation of the catalytic serine and histidine residues are maintained.
No arginine or histidine residues were lost by modification . Discussion.
In some embodiments, the peptide tag comprises at least one to six histidine residues.
The domain contains 10 conserved cysteine and histidine residues that probably are zinc ligands.
In general, zinc fingers coordinate zinc ions with a combination of cysteine and histidine residues.
In this figure, UspA2 yield is affected by histidine residues present in the construct.
The method of claim 1, wherein the peptide tag comprises at least three histidine residues.
The fusión polypeptide contained six histidine residues to enable purification using Ni-NTA agarose.
Its structure is globular, with five alpha helices and rich in cysteine and histidine residues.
In a specific embodiment, the lysine and histidine residues of the compound are in L-configuration.
Following this the synthetic oligonucleotide has 18 nucleotides capable of coding for six consecutive histidine residues.
In another specific embodiment, the lysine and histidine residues are in L-configuration.
Herein, the histidine residues act as multifunctional chelators for the ( metal ) radionuclide.
Said His-tag comprises or consists of six histidine residues in length.
The method according to Claim 5, wherein said metal chelating group is six consecutive histidine residues.
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