Examples of 'hybridoma culture' in a sentence
Meaning of "hybridoma culture"
hybridoma culture: This phrase pertains to a technique used in biology and biotechnology to produce hybridoma cells, which are hybrid cells generated by fusing a specific antibody-producing cell with a cancerous cell. This process allows for the continuous production of monoclonal antibodies
How to use "hybridoma culture" in a sentence
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hybridoma culture
Kinetic screening of primary hybridoma culture supernatants.
Immobilized hybridoma culture supernatants were reacted with MAdCAM.
Thermodynamic screening of secondary hybridoma culture supernatants.
Conditioned hybridoma culture supernatants containing the desired antibodies are collected.
Antiserum of immunized mouse of supernatant of hybridoma culture was taken as sample.
Conditioned hybridoma culture supernatant containing the antibody homologs is collected.
Hybridomas producing a desired antibody are detected by screening the hybridoma culture supernatants.
An equivalent amount of fresh hybridoma culture medium was added to control cultures.
The hybridoma culture supernatant was screened for reactivity against pPSA.
The monoclonal antibody in the hybridoma culture was inspected on the following points.
The hybridoma culture was then expanded in vitro for antibody production.
The strips were then incubated with either mouse serum or hybridoma culture supernatant.
Conditioned hybridoma culture supernatant containing the antibody can then be collected.
The specific monoclonal antibodies can be obtained according to the conventional hybridoma culture method.
The hybridoma culture supernatants described above can be screened by three independent assays.
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Pure mAbs are prepared by protein A sepharose purification of IgG from hybridoma culture supernatants.
MAb were harvested from hybridoma culture supernatant or from mouse ascitic fluid.
To these solutions was added 25 µL of diluted hybridoma culture supernatant.
Hybridoma culture supernatants were screened by RIPA and Western blot analysis.
Indirect ELISA for screening hybridoma culture supernatants.
First, the hybridoma culture has to incubate for ten minutes.
Results of the examination of activities of hybridoma culture supernatants are shown in Fig . 6.
Hybridoma culture supernatant 's antibody titre can be measured by ELISA.
ELISA testing is performed to detect binding of hybridoma culture supernatants to ROR1.
Antisera of test bleeds, hybridoma culture supernatants or ascitic fluids were applied as a primary antibody.
After the culture for 10 to 20-days, the supernatant of each hybridoma culture was collected.
In this Example, hybridoma culture supernatants were tested for the activity of suppressing aPC inactivation.
Particularly preferred is the hybridoma culture designated PMI-1.
Because hybridoma culture supernatant are very dilute, sample volume is increased to 90 µl.
After blotting off the NSS, 50 ul of hybridoma culture supernatant was added per well.
Immobilized hybridoma culture supernatants are then reacted with biotinylated EGFR T790M mutant.
Fig . 4 is a graph showing the inhibition of cynNR10/cynOSMR/BaF3 cell growth by hybridoma culture supernatants.
Twofold dilutions of hybridoma culture supernatants were applied and tested as described previously ( 51 ).
Hybridoma culture medium sample was added to the wells ( 50uL ) and incubated for 1 hour.
Thereafter, wells are incubated with hybridoma culture supernatant, 1/2 diluted in PBSTC.
A hybridoma culture supernatant was positive if the measured optical density ( O.D. ).
Thereafter, wells were incubated with hybridoma culture supernatant, 1/2 diluted in PBSTC.
Hybridoma culture fluids reacting specifically to the immunogen, i.e., HCV protein SEQ.
In the other experiment ( Table IX ), using 5A8 hybridoma culture supernatant, there was no such preincubation.
Ml of the hybridoma culture supernatant was mixed with 0.01 ml Protein G Sepharose ( manufactured by Pharmacia ).
TIB 105 is purified from hybridoma culture supernatants over a recombinant protein G column ( Pharmacia ).
From hybridoma culture supernatants by ( NH4 ) 2S04 precipitation and protein A affinity chromatography.
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Specific training procedures adapted to local culture
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Examples of using Hybridoma
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Each diluted hybridoma supernatant was tested in quadruplicate
For production of monoclonal antibodies by producing hybridoma
Kinetic screening of primary hybridoma culture supernatants
