Examples of 'hybridoma supernatants' in a sentence
Meaning of "hybridoma supernatants"
Hybridoma supernatants: These are the liquid substances found above the sediment in a container holding hybridoma cells, which are used in biomedical research for producing monoclonal antibodies
How to use "hybridoma supernatants" in a sentence
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hybridoma supernatants
Hybridoma supernatants used in both sets of experiments were undiluted.
The experiments included negative control hybridoma supernatants.
Hybridoma supernatants were then harvested by centrifugation.
In a first step a kinetic screening of hybridoma supernatants was performed.
Hybridoma supernatants containing anti HBVsAg antibodies were serially diluted.
Cell surface staining of tumor cell lines with hybridoma supernatants.
Hybridoma supernatants containing anti HBs antibodies were serially diluted.
A high throughput method was developed to process thousands of hybridoma supernatants at one time.
Hybridoma supernatants were then screened for positive clones by immunoprecipitation.
The monoclonal paratopic molecules can be utilized as obtained from hybridoma supernatants or as ascites.
Hybridoma supernatants or dilutions of purified antibody were then incubated in the wells.
Such antibodies may be obtained by screening hybridoma supernatants for the desired inhibitory activity.
Hybridoma supernatants only are reactive with peptides used for immunization.
This plate was used to perform primary and secondary screening of the crude hybridoma supernatants.
As control all hybridoma supernatants were tested against two irrelevant peptides.
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No reaction with Tau was observed for any of the hybridoma supernatants tested.
This involves testing hybridoma supernatants for binding to each screening ligand.
Proliferation analysis of different cancer cell lines after incubation with hybridoma supernatants for 72 h.
Selected hybridoma supernatants were subsequently tested by western blotting and further analyzed by immunohistochemistry.
Table I compares the data obtained with the IPSA for hybridoma supernatants and purified antibodies.
Hybridoma supernatants were tested against the respective peptide used for immunization of mice.
For two rounds of screening the hybridoma supernatants were tested only against the cancer cells.
Hybridoma supernatants obtained from the fusión are tested by any of the known immunoassay methods.
Flow cytometry Serum and hybridoma supernatants were tested using flow cytometry.
Hybridoma supernatants were primarily tested on frozen sections of human lymph nodes using immunoperoxidase staining.
This data indicates that several hybridoma supernatants contained anti-NGF antibodies.
The hybridoma supernatants were then normalized for IgG levels and their inhibitory activities compared.
All antibodies were obtained from serum-free hybridoma supernatants and purified by ammonium sulfate precipitation.
Hybridoma supernatants were also tested in a sandwich RIA.
Example 5, Binding characteristics of hybridoma supernatants and monoclonal antibodies.
Undiluted hybridoma supernatants were incubated on the plates for one hour at RT.
An example of functional activity screening of hybridoma supernatants is shown in Figure 4.
Screening of the hybridoma supernatants revealed two colonies producing antibodies reactive with the coating antigen.
An example of functional activity screening of hybridoma supernatants is shown in FIG . 4.
All hybridoma supernatants were tested in ELISA for specific binding to these peptides.
ZZ polyester beads, An efficient and simple method for purifying IgG from mouse hybridoma supernatants.
The hybridoma supernatants were screened for antibody production using an ELISA assay.
Incubation continued for an additional 4-7 days prior to screening the hybridoma supernatants for neutralizing activity.
Hybridoma supernatants from this fusión were screened for reactivity with PHA blasts.
ELISA results for initial hybridoma supernatants are shown in Table 2 below.
Hybridoma supernatants initially screened by ELISA.
After cultivation for 2 weeks, hybridoma supernatants from 17 wells showed a strong positive reaction.
Hybridoma supernatants were screened for ErbB2-binding by ELISA and radioimmunoprecipitation.
Figures 3, Kinetic ranking assays on antibody-producing hybridoma supernatants.
Purified hybridoma supernatants each were tested in the ELISA for specific binding to all peptides.
Cell surface staining of Ovr110 expressing cells with hybridoma supernatants.
Screening for positive hybridoma supernatants is performed with the bioassay for OCF described herein.
Shows western blots of HEK293 cells with different hybridoma supernatants.
The hybridoma supernatants obtained from the fusion are screened by Western Blotting.
Western blot for determining the specificity of antibodies raised against GT468 in hybridoma supernatants.
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Examples of using Supernatants
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The culture supernatants were screened as described above
The difference is very significant in the supernatants
The resulting supernatants were dried under vacuum
Examples of using Hybridoma
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Each diluted hybridoma supernatant was tested in quadruplicate
For production of monoclonal antibodies by producing hybridoma
Kinetic screening of primary hybridoma culture supernatants
