Examples of 'kinase assay' in a sentence
Meaning of "kinase assay"
kinase assay - a scientific technique used to measure the activity or concentration of kinases, enzymes that catalyze the transfer of phosphate groups to specific substrates in cellular processes
How to use "kinase assay" in a sentence
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kinase assay
The kinase assay was conducted as described herein.
Techniques for quantitative kinase assay are well known in the art.
Kinase assay included the following steps.
No extract exhibited a kinase assay value without the enzyme extract.
The final pool is assayed for activity in a phosphoinosititol kinase assay.
The cell free kinase assay can be carried out as follows.
Two groups of individuals were selected for the in vitro kinase assay studies.
Kinase assay protocols well known to those of ordinary skill in the art were used.
The final pool is assayed for activity in the phosphoinostitol kinase assay.
The human Rho kinase assay was performed as follows.
A proposed assay format for the fluorescent kinase assay is given below.
In vitro kinase assay showed that Snail was phosphorylated by ATR as shown in FIG.
Protein activity may also be determined by an in vitro kinase assay or binding assay.
In vitro MAP kinase assay Inhibitory activity was also confirmed in direct assays.
SAPK activity can also be determined by an in vitro kinase assay.
See also
The Antibody Beacon tyrosine kinase assay kit was purchased from Life Technologies.
MLK protein activity can also be determined by an in vitro kinase assay.
In vitro MAP kinase assay.
The kinase assay used measures the conversion of a fluorescent peptide ( substrate ) to a phosphorylated product.
Whole cell MAP kinase assay.
The activity of growth factor regulated PI3Ks can also be measured using a lipid kinase assay.
If you could design the ideal kinase assay system what would it look like?
Then the resulting GST-IkBa fusión protein was purified and biotinated for kinase assay.
For example, the skilled person can implement the kinase assay provided in example 2 below.
Kinase Assays The kinase assay was performed in 96 well plates at room temperature.
The RET kinase-inhibiting activity can be determined by cell free kinase assay.
LKB1 Assay LKB1 kinase assay is conducted using standard conditions for kinase assays.
The purified KDR core kinase was active in the kinase assay described herein . EXAMPLE 4.
Figure 12 ( c ) assesses the kinase activity of three mutants by immune complex kinase assay.
The Kinase-Glo ® Luminescent Kinase Assay can be used with virtually any kinase and substrate combination.
Ceramide was quantified using the diacylglycerol ( DG ) kinase assay.
EXAMPLE 12 In vitro MAP kinase assay Inhibitory activity can be confirmed in direct assays.
Immunoprecipitates are then resuspended in 40 µl of PI3 kinase assay buffer.
As above-mentioned, in vitro kinase assay are well-known to the skilled person.
Biological activity of cyclin E2/cdk2 was evaluated using a kinase assay.
Following immunoprecipitation of S6K1, a standard kinase assay is performed as described above.
The kinase assay is performed essentially as described for p38a MAP kinase.
This also was reflected in an in vitro kinase assay performed on the immunoprecipitates ( FIG . 2B ).
PI3Ka PI3Ka activity was measured using ADP-Glo luminescent kinase assay.
Following immunoprecipitation of S6K1, a standard kinase assay can be performed as described above.
Table 3 shows IC50 values of several compounds of the invention using LanthaScreen ™ kinase assay.
The in-vitro kinase assay used for MNK1 activity was a homogeneous time resolved fluorescence ( HTRF ) assay.
The kinase inhibitory activity Compound 19 was tested using the kinase assay described in Example 3.
The in-vitro kinase assay used for MNK2a activity was a homogeneous time resolved fluorescence ( HTRF ) assay.
The kinase activity of cells was measured by a Kinase-Glo ® Luminescence Kinase Assay ( Promega ).
Example 7, Kinase assay Kinases are key targets in drug discovery.
The IC50 value was determined with the aid of a biochemical ATM kinase assay.
Perform the ERK1 kinase assay in vitro using a TR-FRET assay.
Appropriate fractions were pooled, total protein determined, and submitted for kinase assay . CDK2 Kinase Assay.
Protocol A radiometric protein kinase assay ( 33PanQinase ® Activity Assay ) is used for measuring the kinase activity.
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