Examples of 'kinase buffer' in a sentence
Meaning of "kinase buffer"
kinase buffer ~ Definition: A solution or substance used in biochemistry to maintain the stability and activity of kinases, enzymes that catalyze the transfer of phosphate groups. Context: This phrase is frequently used in scientific research or laboratory settings when working with kinase enzymes
How to use "kinase buffer" in a sentence
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kinase buffer
The pellets were suspended in kinase buffer to give a standard enzyme product.
The immunoprecipitates were washed twice with lysis buffer and twice with kinase buffer.
The appropriate volume of kinase buffer and inhibitor sample was added to the tube.
The substrate is thawed on ice and diluted to 2 µM in kinase buffer.
The appropriate volume of kinase buffer and inhibitor sample is added to the tube.
The substrate was thawed on ice and diluted to 2 uM in kinase buffer.
Ul Kinase buffer in all rows necessary.
Compartment A contains a creatine kinase buffer solution made as set forth above.
The substrate was thawed on ice and diluted to 2 µM in kinase buffer.
The compounds were diluted in 1 × kinase buffer at the appropriate concentration.
A magnet is used to sediment the beads and the tube is washed with 1X kinase buffer.
Add 80 µl working kinase buffer mix to ELISA plate containing captured PDGFR.
Exogenous substrate was added and then complete kinase buffer was added,.
Negative control, add 40 µl kinase buffer plus 10 µl kinase buffer without ATP.
Kinase activity is determined at 30 °C in the kinase buffer.
See also
Thus, four creatine kinase buffer solutions, numbered 1 through 4, were prepared, as set forth by Table 4.
For negative controls, add 20 µL 2x kinase buffer without enzyme.
A phosphopeptide standard curve ( 0 - 10 nM ) using kinase buffer as the diluent was also tested.
Briefly, proteins and 500 µM ATP were incubated in kinase buffer for 20 min at room temperature.
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Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
Examples of using Kinase
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Creatine kinase can be checked if myalgias develop
Blood tests show elevated creatine kinase
Elevation of creatine kinase in blood is indicative of myositis
