Examples of 'kpn' in a sentence
Meaning of "kpn"
KPN stands for 'Kuwait Petroleum Corporation' - a multinational petroleum company based in Kuwait, known for its activities in the oil and gas sector
How to use "kpn" in a sentence
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kpn
Insertion of the annealed oligos resulted in loss of the Kpn I site upon insertion.
Digestion of these plasmids with Kpn I dissects the pneumococcal inserts from the parent vector.
The plasmid obtained was digested with Kpn I and SwaI.
This introduced the Kpn I site at the end of LC variable domain.
The amplified DNA-fragment and plasmid pDAB5 were restricted with the endonucleases EcoRI and Kpn I.
The plasmid was linearized with Kpn 1 before electroporation into ES cells.
The Kpn I site is represented by the base number 1.
Digest the plasmid obtained in step 14 with Kpn I and isolate the largest fragment.
Thereafter, the Kpn I was removed by treatment with phenol, followed by precipitation with ethanol.
When the chromosome was digested with Kpn I, K8 plasmid was rescued as such plasmid.
The Kpn I site was formed by use of standard site-specific mutagenesis.
An Eco RI fragment of 7 kb and a Kpn I fragment of 7 kb hybridized with the probe.
Instead, a new Sal I site was introduced directly downstream of the Kpn I site in exon 1.
Kpn hotspot ( only free with kpn subscription ).
The DNA fragment was ligated with pVK7 having been digested with a restriction enzyme Kpn I.
See also
The fragment was inserted into a Kpn I site of pSTV28-rpoH to obtain pRPH.
Correct clones were identified by the presence of a 1.2 kb Kpn I fragment.
A former Kpn I restriction site ( GGTACC ), underlined above, has been eliminated by this codon alteration.
This plasmid was generated to produce a 40 kD thioredoxin - Kpn I methylase fusión protein.
In particular, a Kpn I fragment of 4.5 kb was present in all cases.
Specifically, the plasmid pHSG-gdh+gltA was digested with a restriction enzyme Kpn I.
Individual isolates were screened for the presence of the 1.2 kb - Kpn I fragment by restriction analysis.
In addition, the Kpn I lane shows a 0.5 kb band which was better seen on other blots.
The secreted alkaline phosphatase gene was isolated as a 1.8 kb Hind III to Kpn I fragment.
This linear DNA was cleaved with Kpn I to produce a suitable complementary " sticky-end ".
Three micrograms of plasmid pAC373 was cleaved with the restriction endonuclease Kpn I ( Bethesda Research Laboratories ).
The rest of pGEM-4Z, including the Kpn I and EcoR I cloning sites, remained intact.
All of these recombinant clones contained a plasmid ( pMR-C ) with a 7 kb Kpn I insert.
The synthetic gene contained flanking Bam HI and Kpn I sites to facilitate cloning into pTB210.
The structural gene for catalase-peroxidase was subsequently localized to a 2.5 kb EcoRV - Kpn I fragment by sub-cloning.
This cDNA construct was then ligated into the Kpn I and Hind II sites of pUC19.
Correct plasmid was identified by the presence of a 1.45 Kpn I digestion fragment.
The hygR gene was then removed by digestion with Kpn 1 resulting in the vector pUP410.
The plasmid pCGP806 was restricted with Bam HI and Kpn I to release the cDNA insert.
A second digest of pAB0018 with Pmel and Kpn was then performed.
The resulting vector, designated Zem238, contains a Kpn I site after the finger domain.
