Examples of 'kpni' in a sentence
Meaning of "kpni"
Kpni is a noun that stands for a type of restriction enzyme used in molecular biology
How to use "kpni" in a sentence
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kpni
This primer also contained a KpnI site.
Through incorporation the KpnI restriction site disappeared.
The orientation is tested by digestion with KpnI.
This KpnI end is adjacent to and downstream of a BstEII site.
Containing restriction sites KpnI and MluI.
The KpnI site is used as a diagnostic marker for the insertion.
The construct was digested with BamHI and KpnI.
The KpnI fragment was generated by digestion with the restriction enzyme.
Note the presence of SalI and KpnI sites.
Restriction sites KpnI and XhoI served as insertion sites for heterelogous genes to be expressed.
This fragment was digested with KpnI and BamHI.
Map identifies KpnI restriction site used for Southern analysis.
These clones were digested with KpnI and BamHI.
Hybridizing EcoRI and KpnI fragments were subcloned and subjected to DNA sequence analysis.
The construct is digested with SalI and KpnI.
See also
This fragment was synthesized with flanking KpnI and SacI sites to facilitate cloning.
The recovered fragments were then digested with KpnI.
Primers carry either the NdeI and KpnI or the KpnI and XbaI restriction sites.
The new restrictive enzymes used were BglII and KpnI.
The positions of the EcoRI and KpnI sites on the genomic DNA are arbitrary.
The intermediate plasmid was treated with KpnI and HindIII.
End Rescue was performed with KpnI on three fragmented YACS of this size.
The interfaces used for cloning were KpnI and EcoRI.
KpnI Cleavage site of the restriction enzyme KpnI.
These obtained fragments were treated with KpnI and ligated.
In addition, a KpnI site was introduced after the SpeI site.
This introduced an NdeI site immediately downstream of the KpnI site.
The KpnI - BamHI fragment was then cut with HindIII.
The pUCgapP obtained was digested with restriction enzymes EcoRI and KpnI.
PCR was performed with primer pairs encompassing KpnI and Bsml restriction sites.
An internal fragment was released by digesting the DNA with KpnI.
Digesting the nucleic acid molecules with a KpnI restriction enzyme ; and.
The cap gene PCR products are then digested with KpNI.
This approach involves the same 15 kb KpnI fragment used in the previous approach.
The plasmid with the insert in the correct orientation was initially determined by KpnI digestion.
In addition, a KpnI site was introduced after the XbaI site for subsequent subcloning.
The resulting amplicon was digested with BamHI and KpnI and gel purified.
At the KpnI side, on the other hand, we found that significant deletions were tolerated.
This was then inserted into the TA vector cleaved with KpnI.
Degenerate primers containing EcoRI and KpnI sites and further cloned into pUC 18.
This DNA fragment was treated with restriction enzymes KpnI and XbaI.
Plasmid pBAK21 was also digested with KpnI and BamHI and the vector fragment was isolated.
The natural translational initiation of murine WAP is located just downstream of the KpnI site.
The KpnI and SacI ends of the BamHI / KpnI and SacI / BamHI fragments were phosphatased.
The resulting PCR product which contained a chimeric env gene was cleaved by KpnI and NotI.
The polylinker between KpnI and EcoRV was deleted removing the endogenous SalI site, generating pME4.
The DNA is further digested with KpnI.
These were digested with KpnI and probed with a Vλ1 probe.
DNAs were dissolved in TE buffer and cleaved with KpnI.
The resulting fragment was subcloned into pBluescript in the KpnI and BamHI sites, creating pBS-Nde.
