Examples of 'labeled nucleotide' in a sentence
Meaning of "labeled nucleotide"
labeled nucleotide: A nucleotide that has been marked or identified in some way, usually for the purpose of tracking it in an experiment or study
How to use "labeled nucleotide" in a sentence
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labeled nucleotide
A labeled nucleotide obtainable by a method according to any of the preceding claims.
No significant bias with respect to the incorporation of labeled nucleotide was observed.
Extension incorporates a labeled nucleotide thereby generating a labeled extended primer.
Polynucleotides can be labeled either during synthesis or by incorporating at least one labeled nucleotide.
This labeled nucleotide is then advantageously utilized for the synthesis of labeled oligonucleotides.
Proliferation is quantified by measuring the amount of labeled nucleotide taken up by the cells.
The labeled nucleotide is used as a probe for detecting a target nucleic acid in amplification reactions.
The invention of course also encompasses a labeled nucleotide obtainable by a method as disclosed above.
The sequencing reaction begins by introducing a DNA polymerase and a fluorescently labeled nucleotide.
Those sites where primer extension causes a labeled nucleotide to be incorporated can be detected.
Then a complementary 60 bp oligonucleotide was hybridized with the labeled nucleotide.
The procedure produces a labeled nucleotide probe having a target recognition moiety and a signal generating moiety.
The housing is constructed to facilitate identification of labeled nucleotide analogs positioned on the support.
For example, a labeled nucleotide may be added to a test culture medium.
Labeling is usually performed during reverse transcription by incorporating a labeled nucleotide in the reaction mixture.
See also
However, in other examples, labeled nucleotide bases are added as an oligonucleotide.
Point mutations can be identified by hybridizing amplified DNA to labeled nucleotide sequences.
In some examples, labeled nucleotide bases are added as single bases.
The method for detecting the RNA is selected based on the labeled nucleotide analog employed.
In other embodiments, the labeled nucleotide analog is a radioactively labeled nucleotide.
In a separate embodiment, transcription amplification, as described above, using a labeled nucleotide e . g.
In some examples, the added labeled nucleotide base is a set of labeled nucleotide bases.
Labeled nucleotide analogues, such as Cyc3-dUTP may also be used to facilitate detection.
In a third aspect, the invention includes a labeled nucleotide having the formula,.
A labeled nucleotide probe formed according to the chemical synthesis having the following steps,.
Also, some example methods include adding the labeled nucleotide base as a single base.
A labeled nucleotide probe comprising,.
When the snp is present, the eTag reporter labeled nucleotide will be released for detection.
A labeled nucleotide according to claim 20, wherein the spacer is an oligolysine or a polylysine.
A method according to Claim 15 wherein the labeled nucleotide is radiolabeled dGTP.
Unincorporated labeled nucleotide appears as a dense signal near the bottom ( " NTPs " ).
An RNA fragment according to claim 11, wherein the labeled nucleotide is a thiophosphate nucleotide.
The labeled nucleotide was joined to synthetic 20-nt antisense siRNA by T4 RNA ligation of 32pCp.
The method of claim 49 wherein the labeled nucleotide contains a fluorophore.
In some examples, each added labeled nucleotide base is identified between each subsequent sequencing reaction.
Figures 4A and B schematically show labeled nucleotide tetraphosphates according to certain embodiments of the invention.
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