Examples of 'labeled oligonucleotide' in a sentence
Meaning of "labeled oligonucleotide"
A labeled oligonucleotide is a short sequence of nucleotides (DNA or RNA) that has been chemically modified to include a detectable label, such as a fluorescent dye or radioactive element. Labeled oligonucleotides are commonly used in molecular biology techniques like DNA sequencing, PCR, and in situ hybridization for visualizing or detecting specific nucleic acid sequences
How to use "labeled oligonucleotide" in a sentence
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labeled oligonucleotide
A labeled oligonucleotide probe be derived synthetically or by cloning.
The present invention relates to a novel labeled oligonucleotide.
Significant quenching of labeled oligonucleotide probes was observed at all temperatures.
In one embodiment an oligonucleotide hybridizing with a terminal extension is labeled oligonucleotide.
The labeled oligonucleotide was dialyzed against tris buffer solution.
Also disclosed are detectably labeled oligonucleotide primer and probe molecules.
A labeled oligonucleotide can be used as a probe to detect the presence of a nucleic acid.
Concentration labeled oligonucleotide.
The labeled oligonucleotide contained one europium chelate per each oligonucleotide molecule.
Ligation permits the recovery of the labeled oligonucleotide by using avidin.
At least one labeled oligonucleotide probe specific for said target nucleic acid.
Over a period of time an accumulation of a specifically labeled oligonucleotide probe was achieved.
A method using a labeled oligonucleotide of the present invention as a labeled probe.
This particular embodiment makes it possible to obtain a labeled oligonucleotide of molecular beacon type.
A single labeled oligonucleotide may be responsible for both capture and detection of the target.
See also
Preferably the aforementioned probe is a labeled oligonucleotide complementary to the nucleic acid.
The labeled oligonucleotide will include a sequence complementary to the second oligonucleotide units of the multimer.
Also described herein are detectably labeled oligonucleotide primer and probe molecules.
Targets can be reliably imaged through the application of multiple short singly labeled oligonucleotide probes.
Such diagnostic kits comprise a labeled oligonucleotide which encodes portions of the gene transcript.
The efficiency is given in percentage of total cells that contain the labeled oligonucleotide decoy.
The assay may also employ labeled oligonucleotide to allow ease of identification in the assays.
The bead bound PCR product is then hybridized with a chemiluminescent labeled oligonucleotide.
The invention also provides detectably labeled oligonucleotide primer and probe molecules.
The labeled oligonucleotide as claimed in claim 1, wherein said quencher is dabsyl.
This quenching occurs without hybridization of the labeled oligonucleotide to its complementary sequence.
The labeled oligonucleotide as claimed in claim 1, wherein said fluorophore is fluorescein.
This quenching occurs in solution without hybridization of the labeled oligonucleotide to its complementary sequence.
The labeled oligonucleotide was used to prepare a solid phase & affinity test sensor.
DNA was genotyped using one fluorescently labeled oligonucleotide primer and one unlabeled oligonucleotide primer.
The plaques were screened for GRP receptor DNA sequences with labeled oligonucleotide probes.
The present invention includes labeled oligonucleotide which are useful as probes for performing oligonucleotide hybridization.
Methods for in-solution quenching of fluorescently labeled oligonucleotide probes.
Purification The FAM labeled oligonucleotide was purified using reverse phase HPLC.
Importantly, the target is now free to react with an intact labeled oligonucleotide.
In certain preferred embodiments the labeled oligonucleotide probe has a length of up to 22 nucleotides.
In some embodiments, the first moiety may be a labeled probe, such as a labeled oligonucleotide.
The process of claim 1, wherein said labeled oligonucleotide is labeled internally.
Detecting and / or measuring the signal generated by the hydrolysis of the labeled oligonucleotide.
The kit of claim 1, wherein said labeled oligonucleotide is not coupled to a membrane.
In another embodiment, said sequencing comprises allele specific hybridization to a library of labeled oligonucleotide probes.
In this assay, a labeled oligonucleotide is employed that is specific for a target sequence.
Alternatively, the detection reagent is a water soluble, labeled oligonucleotide useful in a hybridization assay.
Kits include the labeled oligonucleotide probe or, if unlabeled, specific labeling reagents may be included.
The signal generated by the hydrolysis of the labeled oligonucleotide is detected and / or measured.
The labeled oligonucleotide is then purified by polyacrylamide gel electrophoresis ( PAGE ).
A method for analyzing a mixture of labeled oligonucleotide fragments of different lengths comprising,.
Colonies containing the IL-4 cDNA insert were selected using a labeled oligonucleotide probe.
The kit of claim 1, wherein said labeled oligonucleotide contains at least two labels.
The labeled oligonucleotide ( 10 ul ) is added to the membranes.
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