Examples of 'ligand-binding' in a sentence
Meaning of "ligand-binding"
In the context of biology and biochemistry, ligand-binding refers to the process of a ligand binding to a receptor molecule
How to use "ligand-binding" in a sentence
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ligand-binding
The receptor can be a ligand-binding fragment of a receptor.
Dimerization or multimerization may provide increased ligand-binding affinity.
Ligands and ligand-binding moieties are paired affinity tags.
The same is true of the ligand-binding domain.
The ligand-binding functions are predicted to reside in the extracellular domain.
Assessment of changes in ligand-binding affinities.
Ligand-binding assays are used to determine interaction with the receptor of interest.
All of these molecules have an extracellular ligand-binding domain.
A receptor ligand-binding polypeptide can also be used for purification of ligand.
Initial crystallography studies focused on the ligand-binding enzymatic processes.
The ligand-binding properties and signaling mechanisms of these receptors are remarkably similar.
These are examples of positions which are proximal to the ligand-binding pocket.
Affinity gels typically consist of a ligand-binding moiety immobilized on a gel support.
A preferred fragment produces an antibody that diminishes or completely prevents ligand-binding.
The ligand-binding domain is positioned on the extracellular side of the plasma membrane.
See also
This can be used to change the specificity of the ligand-binding pocket.
Simple ligand-binding studies will not distinguish between these two classes.
Binding conditions are those that maintain the ligand-binding activity of the receptor.
The ligand-binding site is located at the interfaz between the two domains.
The latter two examples show attachment proximal to the ligand-binding pocket.
Ligand-binding assays can be used to detect binding of test compounds to particular sequences.
In an alternative embodiment, the therapeutic molecule is a ligand-binding protein with biological activity.
The ligand-binding site is located at the interfaz between the bPBP 's two domains.
In preferred aspects, the receptor protein is a polypeptide chain of a ligand-binding heterodimeric receptor.
The extracellular ligand-binding domain can be specific for any antigen or epitope of interest.
In preferred embodiments, the receptor protein is a polypeptide chain of a ligand-binding heterodimeric receptor.
A particularly preferred ligand-binding heterodimeric receptor utilizes a prokaryotic secretion signal as described herein.
The ligands used are capable of binding to two or more of the ligand-binding domains.
More particularly, more than one ligand-binding domain is used in the expression construct.
In a second series of experiments, amino acid substitution mutations were targeted to ligand-binding domains.
The ligand-binding sites of the receptors are located to the three first immunoglobulin-like domains.
The present invention provides an isolated polynucleotide encoding a ligand-binding receptor polypeptide.
The receptor protein molecule has a ligand-binding site on its extracellular region or soluble region.
The present invention can be used to generate a signal from a variety of ligand-binding domains.
A soluble receptor includes the extracellular, ligand-binding domain of the corresponding cell surface receptor.
Such assays are well known and widely used for assessment of ligand-binding to GPCRs.
Similarly encompassed are ligand-binding antibodies that inhibit receptor activation without inhibiting receptor binding.
One such method is to perform limiting dilution of the complex pool of ligand-binding molecules.
Once the structure has been generated, potential ligand-binding regions are identified by the computer system.
Accordingly, ligand-binding regions of different sizes comprising different extracellular domain structures may be amplified.
The multi-spanning transmembrane protein comprises a ligand-binding domain which binds to an extracellular ligand.
Ligand-binding receptor polypeptides can also be used within other assay systems known in the an.
Thus, a preferred embodiment contemplates a ligand-binding heterodimeric receptor comprising first and second polypeptides.
Ligand-binding receptor polypeptides can also be used within other assay systems known in the art.
Soluble receptors are most commonly ligand-binding receptor polypeptides that lack transmembrane and cytoplasmic domains.
Properdin is a gamma globulin protein composed of multiple identical protein subunits with a separate ligand-binding site.
These extracellular domains are useful for in vitro ligand-binding assays, both soluble and solid phase.
Oligonucleotide primers may be used to amplify nucleic acid fragments encoding taste receptor ligand-binding regions.
A hybrid receptor will be created with a ligand-binding domain and DNA-binding domain from different sources.
Other forms of detecting and quantifying the protein include affinity chromatography techniques, ligand-binding assays, etc.
