Examples of 'ligase' in a sentence
Meaning of "ligase"
Ligase is a noun that refers to an enzyme that is capable of catalyzing the joining of two large molecules by forming a new chemical bond
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- Any of a group of enzymes that catalyze the binding of two molecules; synthetase.
How to use "ligase" in a sentence
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ligase
The ligase may also be a thermostable ligase.
Parkin is one of a family of ubiquitin ligase enzymes.
Ligase is added and the mixture incubated.
Ligations are preferably conducted by means of a ligase enzyme.
Adding ligase to ligate said hybridized portions.
The mixed fragments were ligated using a ligase.
The ligase can also be a thermostable ligase.
Intramolecular ligation may be effected using a ligase.
A ligase chain reaction also may be utilized.
An example of purification of the ligase is given below.
The ligase was then heat inactivated.
The enzyme may be a ligase or a polymerase.
A ligase may be obtained from recombinant or natural sources.
It also possess ubiquitin ligase activity.
A biotin ligase is needed for activation of these proteins.
See also
These tasks are carried out by the enzyme ligase.
Ubiquitin ligase inhibitors are being developed forthe treatment of cancer.
The enzyme that catalyzes ligation is called ligase.
The ligase optionally has good ligation fidelity.
Target sequences are then ligated together with a ligase.
A ligase enzyme is then used to link the oligonucleotides.
This may also be accomplished using just a ligase.
Ligase covalently seals extended target to second linker.
This includes reactions catalyzed by polymerase or ligase activity.
Any suitable ligase can in principle be used in this step.
Ligation is brought about by ligase enzymes.
Ligase chain reaction.
This is due to inactivation of the host ligase by NaOH.
Itch is an ubiquitin ligase involved in protein recognition and degradation by the proteasome.
In some embodiments the enzyme is a ligase.
Use of ligation fidelity of thermostable ligase to ligate correct tag to target strand.
Incorporation may be mediated by enzymes such as polymerase or ligase.
Ligation is caused by ligase enzymes.
The specificity of the reaction was tested using a reaction without ligase.
The increased expression of ligase activity may be accomplished by any suitable way.
Topoisomerases are enzymes with both nuclease and ligase activity.
Ligase discrimination can be further enhanced by employing various probe design features.
The fragments are then sealed together by an enzyme called ligase.
This method uses a ligase enzyme to join preformed nucleic acid probes.
The ligation is performed by a ligase.
Other amplification methods such as ligase chain reaction may also be employed.
A coupling is strengthened substantially if it is reinforced by treatment with a ligase enzyme.
A ligase is added to couple the codon oligo to the nascent bifunctional complex.
The enzyme can be a ligase.
Ligase is added to the hybridized probes and exposed to standard ligase conditions.
The left lanes depict results in the absence of ligase.
The procedure wherein the ligase is used may be performed as a separate reaction.
The sticky ends can be reattached by another enzyme called ligase.
A modulator that specifically affects ubiquitin ligase activity is a ubiquitin ligase modulator.
Subtilisin variants are disclosed which have an improved peptide ligase activity.
