Examples of 'linearized plasmid' in a sentence
Meaning of "linearized plasmid"
linearized plasmid: A type of plasmid DNA that has been modified to have a linear structure, often used in genetic engineering
How to use "linearized plasmid" in a sentence
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linearized plasmid
One µg of linearized plasmid was added for each reaction.
The filled boxes represent the transcribed region of each linearized plasmid.
The linearized plasmid was then purified and religated.
The gene is then inserted into this linearized plasmid by ligating the two DNAs together.
The linearized plasmid was treated with calf intestinal phosphatase.
Shearing forces may also cut the plasmid DNAs and produce linearized plasmid DNAs.
The linearized plasmid was then cut with NotI and ligated.
The control RNA spikes were produced by in vitro transcription of linearized plasmid templates.
One transformation using the linearized plasmid yielded several hundred transformants.
The linearized plasmid was then treated with calf alkaline phosphatase to prevent recircularization.
PEm was digested with BamHI and the linearized plasmid was digested with calf intestinal phosphatase.
The linearized plasmid was ethanol precipitated and isolated from the supernatant by centrifugation.
The DNA used is linearized plasmid described.
The linearized plasmid is digested with HpaI and the vector fragment recovered.
The HCV cDNA is ligated into this EcoRI linearized plasmid DNA.
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Then the linearized plasmid was used as a template for in vitro transcription as described herein.
Full length mRNA was transcribed from linearized plasmid DNA using a Promega Riboprobe Kit.
Linearized plasmid DNA control is typically used as a nucleic acid internal control.
Probes were synthesized from linearized plasmid template using phage T7 RNA polymerase.
The linearized plasmid was treated with Calf-Intestine Alkaline Phosphatase to remove the 51 phoshate groups.
Following the pre-incubation the linearized plasmid was transcribed as detailed in Example 29.
The linearized plasmid was introduced into RBL and 293 cells by electroporation.
Capped-cRNAs were synthesised in vitro from the linearized plasmid by using the T7 RNA polymerase ( Stratagene ).
SpeI linearized plasmid DNA was used as template for in vitro transcription.
Approximately 500 ng of each linearized plasmid was transfected.
The linearized plasmid was purified and used to transform strain SWE23-DL1 using the LiOAc method.
An aliquot ( 5 µg ) of LA1A2J was cleaved with MluI to produce a linearized plasmid.
The linearized plasmid was used to electroporate the human cell line K562.
As expected, an equal dose of linearized plasmid had no effect on CCI ( Figure 3 ).
The linearized plasmid was gel-purified and recircularized using T ₄ DNA ligase.
Reactions were initiated by the addition of linearized plasmid and incubated for 30 minutes at 37°C.
The linearized plasmid was purified and self-ligated to generate pNC5LSP-5.
The Saccharomyces cerevisiae strain is cotransformed with a linearized plasmid and the isolated p53 fragment from Ad5CMV-p53.
The linearized plasmid is then digested with AatIl and the 2931 bp vector fragment is gel-purified.
A sample of 5 µg of the linearized plasmid is used to transform 1 x 108 spheroplasts.
The linearized plasmid was digested with BAL 31 ;.
The linearized plasmid was then used as a template of transcription reaction ( for 2 hours at 37°C ).
Linearized plasmid is separated from uncut DNA on a 0.6 % agarose gel in Tris-acetate buffer.
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We also calculated the linearized rates of events
A linearized vector may not be a circular plasmid
The customer then produces a linearized amplifier
Examples of using Plasmid
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Said plasmid contains a tetracycline resistance gene
The construction of this plasmid is described below
Deposit of plasmid containing carcinogenic substance adducts
