Examples of 'luciferase reporter' in a sentence
Meaning of "luciferase reporter"
luciferase reporter - In scientific research, a luciferase reporter is a genetically engineered organism or cell line that expresses the luciferase enzyme. This enzyme produces light through a chemical reaction, allowing researchers to track gene expression or other cellular processes by measuring the light emitted
How to use "luciferase reporter" in a sentence
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luciferase reporter
Injection was measured by luciferase reporter gene expression.
The luciferase reporter gene assay was optimized and validated.
Promoter sequences can be tested in luciferase reporter assays.
Furthermore a luciferase reporter gene replaces the nef gene.
Luciferase level was measured by dual luciferase reporter assay.
Luciferase reporter activities were evaluated.
The cells were then subjected to the luciferase reporter assay.
The relative luciferase reporter activity was obtained by normalization to renilla control.
These replicons also contained a firefly luciferase reporter gene.
The luciferase reporter assay was also used to confirm compound potency in the replicon.
Protein expression levels in all luciferase reporter assays were confirmed by western blot.
The signalling cascade induces NFkB activation and allows transcription of the luciferase reporter gene.
Cells incorporating the luciferase reporter gene are selected by including zeocin in the culture medium.
This plasmid contained a perl gene sequence and a luciferase reporter gene sequence.
The recombinant cells harbor luciferase reporter gene under the control of cAMP response element.
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This treatment activates the luciferase gene and causes the production of the luciferase reporter protein.
Use of this luciferase reporter plasmid has been disclosed by Heyman et al.
DNA to lipid ratios are optimized using a luciferase reporter.
A luciferase reporter gene was co-transfected as a control of transfection efficiency.
A MMTV minimal promoter containing ARE was first cloned in front of a firefly luciferase reporter gene.
HCV entry was determined by analysis of Luciferase reporter gene expression as described.
The Notch pathway activation was measured by quantifying Notch signalling driven luciferase reporter assay.
Figure 4 represents the luciferase reporter constructs.
Luciferase reporter assay for NF-kB activation.
Cells were lysed and read on the Berthold MicroLumat Plus for luciferase reporter activity.
Wnt luciferase reporter assay in HeLa cells co-transfected with the indicated constructs.
Cell lines of this type often incorporate a GLI dependent luciferase reporter for the assay readout.
NF-kB luciferase reporter assay activity peaked one hour after placing cells in the CO atmosphere.
Comparison of the antagonistic / inhibitor potencies of various binding agents by luciferase reporter assays.
The cell also contains the firefly luciferase reporter gene controlled by a chimeric IFN-responsive promoter.
In the illustrated example, dsRNA contructs are administered to cells having a recombinant luciferase reporter gene.
Light output was measured by luciferase reporter assay ( Promega ) in a Berthold luminometer.
This cell line is further transfected with a fos promoter-driven luciferase reporter gene construct.
Figure 3A shows the dual luciferase reporter plasmid used for in vitro screens.
As an internal control for transfection efficiency a constitutive renilla luciferase reporter ( promega ) was cotransfected.
Firstly, the luciferase reporter element was constructed and stably transfected into HEK cells.
When the transfected cells are treated with forskolin, luciferase reporter gene expression is induced.
The activity of a luciferase reporter containing mutated TCF binding sites ( FOPFLASH ) was unaffected Fig.
PPAR-Y gene expression was monitored using a luciferase reporter assay system.
Luciferase reporter activity was measured using the Steady-Glo Luciferase Assay Kit from Promega.
Interaction was measured by activation of a luciferase reporter containing five Gal4 binding sites.
We investigated whether overexpression of TAB1 leads to activation of the luciferase reporter gene.
Co-transfection data showing repression of luciferase reporter activity vía VEGF-KRAB protein expression.
Following activation by agonist binding, PPAR induces expression of the luciferase reporter gene.
The replicon also has a firefly luciferase reporter and a neomycin phosphotransferase ( Neo ) selectable marker.
For transactivation, the inventors used an MMTV-driven luciferase reporter system Fig.
The c-fos promoter - luciferase reporter gene constructs and plasmids.
Figure 5A shows a schematic of activation of the hedgehog signaling pathway in the Luciferase reporter assay.
A plasmid comprising a luciferase reporter gene was included in each transfection ( pIND-luc ).
FOXP3 binding regions were cloned between a minP promoter element and a luciferase reporter gene.
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Examples of using Luciferase
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No luciferase expression is observed in this control assay
Injection was measured by luciferase reporter gene expression
Luciferase determination was performed using a chemiluminiscence assay
