Examples of 'luciferases' in a sentence
Meaning of "luciferases"
luciferase (noun) - an enzyme that produces bioluminescence in organisms such as fireflies and certain marine animals. Luciferase is often used in scientific research as a reporter gene to study gene expression
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- plural of luciferase
How to use "luciferases" in a sentence
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luciferases
Synthetic oplophorus luciferases with enhanced light output.
Luciferases have been isolated directly from various sources.
Over time for certain luciferases.
Assays using luciferases are well known in the art.
This may refer to molecules that are substrates for both luciferases and photoproteins.
Mutant luciferases of click beetles are also known in the art.
This combination of ingredients rapidly and effectively quenches luminescent reactions from beetle luciferases.
Specific luciferases are known by both their protein and gene sequences.
The invention further provides nucleic acids which encode the luciferases as described above.
Luciferases are enzymes found in luminous organisms which catalyze luminescence reactions.
Various other reporter applications of luciferases have been described in the scientific literature.
Luciferases and modified mutant or variant forms thereof are well known.
Compositions of the invention include luciferases having greater than the natural level of thermostability.
Luciferases are known to be unstable.
The amino acid sequences of the four luciferases from the ventral organ are highly similar.
See also
Luciferases can be broadly classified into eukaryotic luciferases and prokaryotic luciferases.
The ratio of firefly to Renilla luciferases was calculated.
A number of mutant luciferases were selected from the in vivo assays for thermostability.
The heat stabilisation of Photinus pyralis and Luciola mingrelica luciferases has not yet been reported.
The cDNAs encoding luciferases of various beetle species have been reported.
Data calculation The ratio of firefly to Renilla luciferases was calculated.
Alternatively luciferases may be used such as firefly luciferase and bacterial luciferase.
The following Table III shows spectral properties of these and other exemplary mutant luciferases.
Luciferases are enzymes commonly used as reporters when analyzing molecular events in cells.
Background of the Invention Luciferases are defined by their ability to produce luminescence.
Luciferases can be produced in the lab through genetic engineering for a number of purposes.
More particularly, the invention concerns mutant luciferases of beetles.
The mutant luciferases and DNAs of the invention are employed in various biosensing applications.
Figure 61 is a graphical representation of the resistance of selected luciferases to a substrate inhibitor.
Luciferases can react with a suitable substrate to produce light as one of the reaction products.
Isolated and / or purified luciferases are typically used in the present invention.
Luciferases produce light as an end-product of catalysis.
Ideally, it is preferable that all complexed luciferases are triggered to emit photons simultaneously.
The novel luciferases disclosed herein include variants of the active subunit of a decapod luciferase.
However, the luminescence elicited by luciferases carries a much higher energy content.
The luciferases with the best overall performance were selected after several cycles of this process.
For purposes herein, reference to luciferase refers to either the photoproteins or luciferases.
Other enzymes that are not luciferases can mediate a luminescence reaction using synthetic substrates.
However, as noted above, the physical stability of the luciferases was not identical.
Mutant luciferases of fireflies of genus Luciola are known in the art.
Thus, it would be preferable to further separate the luminescence spectra of the two luciferases.
Preferred luciferases of the invention possess catalytic activity that depends on ATP and emits photons.
These include, but are not limited to, natural and recombinant forms of luciferases and aequorins.
Firefly luciferases are a sub-group of the beetle luciferases.
This system contained two substrates, used to determine the activity of two luciferases sequentially.
The luciferases of beetles can be functionally divided into ph-sensitive or ph-insensitive.
Figure 17 is a diagram showing the evolutionary relationships among beetle luciferases based on amino acid sequences.
Luciferases generate light vía the oxidation of enzyme-specific substrates, called luciferins.
However, cDNA 's coding for other luciferases are also suitable including modified forms of luciferases.
Luciferases are enzymes that catalyse the oxidation of a substrate, luciferin, and produce an unstable intermediate.
