Examples of 'meganuclease' in a sentence
Meaning of "meganuclease"
Meganuclease (noun) - a type of enzyme that recognizes and cuts DNA at specific nucleotide sequences. Meganucleases are often used in genetic engineering and gene editing technologies due to their precision in targeting specific genes. Their ability to induce DNA breaks at specific sites is valuable in research and biotechnology
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- An endonuclease that binds to a specific long nucleotide sequence when cleaving.
How to use "meganuclease" in a sentence
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meganuclease
The assayed meganuclease is introduced in an expression cassette.
Preferably the enzyme is a homing endonuclease or a meganuclease.
Producing a recombinant meganuclease comprising said polypeptide sequence.
The same rules of design are applied to this kind of meganuclease.
The meganuclease are also commonly called homing endonuclease.
Disclosed is the use of recombinant meganuclease for gene therapy.
The assayed meganuclease is incubated with the polynucleotide substrat in appropriate conditions.
Two general methods can be applied to produce such a meganuclease.
Rationally designed meganuclease with altered dimer formation affinity.
The disclsosure describes that the nuclease agent is a meganuclease.
A gBlock was designed to introduce a meganuclease recognition site into the construct.
A second nucleic acid sequence encoding a second recombinant meganuclease monomer.
Same principles of hybrid meganucleases apply to the single chain meganuclease.
The white boxes refer to the gene encoding the meganuclease.
Therefore a meganuclease variant is still part of the invention when used in a heteromeric form.
See also
For integration into endogenous immunoglobulin loci a site specific meganuclease may be used.
Meganucleases can be used to cleave meganuclease recognition sites within the coding regions of a polynucleotide.
A very interesting use of the hybrid meganuclease is for targeting a particular genomic locus of interest.
All plasmid substrates were linearized with XmnI or ScaL concurrently with the meganuclease digest.
Meganuclease heterodimers which cleave DNA sequences in yeast genomes.
The invention also relate variants of the hybrid meganuclease according to the present invention.
The white tags refer to the recognition and cleavage site of the assayed meganuclease.
The invention also contemplates the use of such hybrid meganuclease essentially as recognition domain.
This intervening sequence comprises the recognition and cleavage site of the assayed meganuclease.
Meganuclease heterodimers which cleave DNA sequences in pathogen genomes.
Aspects of the invention allow for the use of recombinant meganuclease for gene therapy.
Meganuclease heterodimers which cleave DNA sequences in plant genomes.
These studies have paved the way for a general use of meganuclease for genome engineering.
Meganuclease heterodimers which cleave DNA sequences relevant to gen therapy.
ZFN was developed to overcome the limitations of meganuclease.
Time course of recombinant meganuclease efficacy in CHO cell reporter assay.
Meganuclease heterodimers which cleave DNA sequences relevant to gene therapy.
Methods for Gene Therapy Disclosed is the use of recombinant meganuclease for gene therapy.
Meganucleases were introduced by transduction of plasmid DNA or mRNA encoding each meganuclease.
In one embodiment, the meganuclease is a homing nuclease.
In yet other methods, the nuclease is a meganuclease.
A single-chain meganuclease has multiple advantages.
Figure 2 is a table disclosing the known meganuclease.
A recombinant meganuclease of claim 1 for use as a medicament.
In another embodiment, the nuclease is a meganuclease.
Wherein said meganuclease is a recombinant meganuclease comprising,.
In another disclosure, the nuclease is a meganuclease.
Such hybrid meganuclease schematically comprises,.
In still another embodiment, the nuclease agent is a meganuclease.
Mating of meganuclease expressing clones and screening in yeast,.
At least one regulatable genomic meganuclease expression construct ; and.
In other embodiments, a genetically-modified anima is produced using a recombinant meganuclease.
A recombinant meganuclease heterodimer comprising,.
In other embodiments, a genetically-modified animal is produced using a recombinant meganuclease.
A recombinant meganuclease monomer comprising,.
