Examples of 'meganucleases' in a sentence
Meaning of "meganucleases"
meganucleases (noun): a type of DNA-cutting enzyme used in genetic engineering and biotechnology
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- plural of meganuclease
How to use "meganucleases" in a sentence
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meganucleases
Most meganucleases are homodimers or internally symmetrical monomers.
The recognition sites of the meganucleases are not palyndromic.
Meganucleases are rather well characterised structurally and mechanistically.
Another class of artificial endonucleases is the engineered meganucleases.
Meganucleases with increased affinity and increased activity.
A database comprising all known meganucleases target sites is prepared.
Meganucleases were used to disable a gene in the livers of rhesus macaques.
We know relatively little about the actual purpose of meganucleases.
The meganucleases could be inteins.
It built up a successful business based on the use of meganucleases in genome engineering.
Meganucleases are therefore considered to be the most specific naturally occurring restriction enzymes.
Same principles of hybrid meganucleases apply to the single chain meganuclease.
Meganucleases can be stably or transiently expressed into cells using expression vectors.
Such targets for hybrid meganucleases can be designed as disclosed above.
A second database is designed comprising all possible targets for hybrid meganucleases.
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The LAMep meganucleases were expressed and purified using the following method.
Disclosed are methods for rationally designing recombinant LAGLIDADG family meganucleases.
Meganucleases method of gene editing is the least efficient of the methods mentioned above.
These new designed endonucleases or meganucleases are hybrid of LAGLIDADG meganucleases.
Meganucleases can be used to cleave meganuclease recognition sites within the coding regions of a polynucleotide.
Double strand breaks can be introduced by meganucleases to raise the occurrence of Homologous Recombination.
Meganucleases were introduced by transduction of plasmid DNA or mRNA encoding each meganuclease.
In one embodiment, the two meganucleases are tethered by a linker.
Engineered meganucleases with recognition sequences found in the human t cell receptor alpha constant region gene.
Number of mono-dodecapeptide meganucleases are known.
Libraries of mutant meganucleases that may contain meganucleases with new nuclease specificity are described.
Number of di-dodecapeptide meganucleases are known.
Single chain meganucleases using the method of Epinat et al.
In preferred embodiments, the nucleases used to practice the invention are single-chain meganucleases.
The making of artificial meganucleases with dedicated specificities would bypass this limitation.
In another aspect, disclosed are the rationally-designed meganucleases resulting from these methods.
The high specificity of meganucleases gives them a high degree of precision and low cell toxicity.
Methods for producing engineered, site-specific recombinant meganucleases are known in the art.
A pair of meganucleases were designed to recognize and cleave the DNA.
Nucleases that can be used include meganucleases and zinc-finger nucleases.
In the wild, meganucleases are essentially represented by homing endonucleases.
In another aspect, disclosed are recombinant single-chain meganucleases resulting from these methods.
A preferred class of meganucleases for use in the present invention is the LAGLIDADG endonucleases.
A robot-assisted mating protocol was used to screen a large number of meganucleases from our library.
Meganucleases constitute a family of very rare-cutting enzymes.
In one embodiment, two meganucleases recognizing adjacent sites are used.
Meganucleases will thus cut cellular genomes only once, at the target locus.
In a preferred embodiment, at least two meganucleases having distinct target sequences are used.
Meganucleases with decreased DNA-binding affinity and decreased activity but increased specificity.
In another embodiment, the first and second meganucleases target constant region gene segments.
Therefore, the meganucleases of the invention have utility in immunotherapy, including cancer immunotherapy.
Embodiments of the invention encompass the recombinant meganucleases described herein, and variants thereof.
Such engineered meganucleases exhibit an extremely low frequency of off-target cutting.
In another aspect, the invention provides the rationally-designed meganucleases resulting from these methods.
Rationally-designed meganucleases with altered sequence specificity and heterodimer formation.
