Examples of 'melting curve analysis' in a sentence
Meaning of "melting curve analysis"
melting curve analysis: A technique used in molecular biology to analyze the melting behavior of DNA during heating
How to use "melting curve analysis" in a sentence
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melting curve analysis
Melting curve analysis was made to ensure specific amplification.
Detection was either by amplification curve or melting curve analysis.
Melting curve analysis can be performed to identify the targets.
Specific amplification was confirmed by a melting curve analysis as above.
Melting curve analysis was also performed for each ChIP primer.
Systems and methods for automated melting curve analysis.
Melting curve analysis is an additional step that can be included in a cycling profile.
The specific of the products was verified by melting curve analysis.
Melting curve analysis required a manual analysis step to be performed by the customer.
Twelve samples were included in each melting curve analysis run.
A melting curve analysis of the products was performed at the end of the amplifications.
The exact description of the melting curve analysis can be found supra.
Melting curve analysis usually begins after the plateau phase of amplification is reached.
Another application of FRET hybridization probes is melting curve analysis.
Amplification was followed by a melting curve analysis to confirm PCR product specificity.
See also
Melting curve analysis may be performed during or subsequent to PCR amplification.
The PCR products are each subjected to a melting curve analysis.
Melting curve analysis of a bridge-probe hybridised to matched and mismatched templates.
The specificity of the PCR reactions was verified by generation of a melting curve analysis.
Alternatively, the melting curve analysis can be performed as an end point analysis.
For example, genotyping can be performed with melting curve analysis.
The practicality and usefulness of melting curve analysis in PCR-based detection systems is well known.
It can also be used in end-point detection and melting curve analysis.
However, the melting curve analysis prior to high-resolution capabilities provided a lack of specificity and accuracy.
Values for denaturation, amplification and melting curve analysis were programmed as follows,.
Accordingly, the amplification reactions can be archived and reevaluated by melting curve analysis.
Melting curve analysis results obtained with lyophilized and ready-to-use amplification mixtures ;.
To control for specificity of the amplification products, a melting curve analysis was performed.
Figure 19 b . Melting curve analysis results obtained with the reference method.
The analysis is performed during the cycling process - no melting curve analysis is needed.
A melting curve analysis was performed as shown in FIG . 9A.
In other embodiments, the testing step can comprise conducting a melting curve analysis.
Figure 4 Real time PCR melting curve analysis according to example 7 Fig.
The Tm of the bridge-probe binding can be determined by melting curve analysis.
Subsequent melting curve analysis was done at a transition rate of 0.2°C/second.
The method of 24 wherein the fluorescence is monitored during melting curve analysis subsequent to amplification.
Melting curve analysis of the 640 nm signal is used to detect MRSA.
Alternatively, the target nucleic acid may be studied through melting curve analysis subsequent to amplification.
The melting curve analysis program has been set up within a range 68 through 95 [ Centigrade ].
After completion of PCR amplification, melting curve analysis was performed.
Melting curve analysis at this rate added 30 seconds to the 8 minute reaction represented in Figure 50.
The kinetic paradigm is more relevant to biochemistry, rapid cycle PCR, and melting curve analysis.
FIG . 9 shows results of melting curve analysis of bridge-probes from examples 2 and 3.
Molecular beacon oligonucleotides can be used for real-time PCR and melting curve analysis.
Melting curve analysis showed only a single product of Tm 84°C.
Instead of FRET hybridization probes, Molecular Beacons may alternatively be used for melting curve analysis.
RT-PCR products were subjected to melting curve analysis using Lightcycler software v3.5.
The method of 41 further comprising the step of performing post-amplification melting curve analysis.
Figure 6, Melting curve analysis of MAGE-A10 PCR products obtained with different primer-combinations for preamplification.
A method according to Claim 1 wherein the determining in step ( c ) makes use of melting curve analysis.
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