Examples of 'melting curves' in a sentence
Meaning of "melting curves"
Melting curves: In science, melting curves refer to graphical representations showing the relationship between temperature and the amount of substance that is in solid phase changing to liquid phase
How to use "melting curves" in a sentence
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melting curves
The background corrected melting curves were then normalized.
Melting curves were highly reproducible.
Amplification specificity was confirmed by the analysis of the melting curves.
Typing was confirmed by analysis of melting curves in the two channels.
Melting curves performed afterwards showed all samples had been digested.
With these dilutions of target the melting curves show two products.
Product melting curves are obtained during a slow temperature increase to denaturation.
The effect of transition rates and dye concentration on melting curves are dye specific.
Screen pictures of melting curves and thermodynamics summary tables were produced.
The novel unbiased hierarchal clustering method is based on a distance metric between melting curves.
These complex melting curves can be used as a fingerprint for product identification.
SNP genotyping was carried out using melting curves analysis.
Melting curves can be analyzed on any suitable software known in the art.
The partial molar heat capacities and melting curves were analyzed using standard procedures.
Melting curves can be used to distinguish products that have different melting temperatures.
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This wide range allows most PCR products to be differentiated by melting curves.
The ability of melting curves to detect genetic polymorphism and mutations will be determined.
Melting onset temperatures were determined from the melting curves obtained from DSC analysis.
Experimental melting curves for near spherical metal nanoparticles exhibit a similarly shaped curve.
Figure 2 shows the amplification curve and melting curves.
Melting curves can also be used for mutation detection during PCR.
The background-corrected melting curves were then normalized.
Probe melting curves are obtained by slowly heating through temperatures around the probe Tm.
In addition, product specificity can be obtained by analysis of melting curves.
Analysis of melting curves can be used to extend the dynamic range of quantitative PCR.
As the concentration of dye was increased, the melting curves were shifted to higher temperatures.
Melting curves were evaluated taking the first derivative with the inflection point indicating the Tm.
Figure 1 reports the melting curves of the antigens tested.
Melting curves with dsDNA dyes can identify heterozygous mutations that produce heteroduplexes during PCR.
For example, fluorescence melting curves have been used to monitor hybridization.
Figure 4 depicts plots of exemplary, unmodified experimental melting curves of hairpin structures.
B Obtained by fitting melting curves to a modified two state model with linear sloping baselines.
Furthermore, unbiased, hierarchal clustering can accurately group the melting curves into genotypes.
The melting curves in FIG.
Monitoring the fluorescent change to define melting curves f i for each target nucleic acid, and.
Melting curves of PCR products were compared on difference plots of temperature-overlaid, normalized melting curves.
Figure 7 shows the melting curves of nevi and melanoma samples.
In accordance with the present invention, an additional use for melting curves is quantitative PCR.
The obtained DSC melting curves after a directed crystallisation are shown in FIG.
Figure 6 depicts derivative plots after exponential background subtraction of the melting curves of Figure 4.
Figure 3 shows the dependence of the melting curves from the concentration of sodium ions.
FIGURE 3 shows the amplification curves and the first derivative of the melting curves of Example 2.
Instead of derivative plots, normalized melting curves can be displayed after exponential background subtraction.
Figure 1A displays the original melting curves.
The present invention 's feature of using melting curves for relative quantification will now be explained.
Melting curves were normalized using exponential background subtraction see U.S. Patent Application No.
However, outside of each melting region, the melting curves of the different genotypes are similar.
The resulting melting curves allow discrimination between primer-dimers and specific PCR product.
Figure 9C shows the clustered genotypes of the normalized melting curves from Figure 9B.
Figure 7B shows the normalized melting curves after exponential background subtraction.
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