Examples of 'minicell' in a sentence
Meaning of "minicell"
minicell (noun) - a small cell, especially a small compartment within a larger structure or organism
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- An achromosomal cell resulting from aberrant cell division, containing RNA and protein but little or no chromosomal DNA.
How to use "minicell" in a sentence
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minicell
Minicell uptake by mammalian cells such as macrophages.
Removal of endotoxin from the minicell preparation.
Minicell uptake and breakdown in macrophage phagolysosomes.
Expression of heterologous protein by minicell transfected macrophages.
Known minicell purification methods also can be combined.
Differential centrifugation of a minicell producing bacterial cell culture.
Minicell compositions and methods.
The segments are then placed into minicell payloads.
We have used minicell foam for many years as well.
Several approaches have been developed to improve conventional minicell purification techniques.
The minicell and ligand may be any of those described herein.
This conditioned the filter for the minicell suspension medium.
The minicell and ligand can be any of those described herein.
Preferred embodiments of the invention exploit bacterial filamentation to increase minicell purity.
Additional embodiments relate to the use of a minicell to deliver the microRNA mimics.
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Particularly effective purification methods exploit bacterial filamentation to increase minicell purity.
The drug can be packaged in a separate minicell from the functional nucleic acid.
The present inventors have further discovered that the removal of endotoxin improves minicell preparations.
Contact between the minicell and the mammalian cell may be in vitro or in vivo.
The residual free endotoxin was removed from purified minicell preparations as follows.
Generation of minicell producing strain from Escherichia coli.
The method optionally includes a treatment of the purified minicell preparation with an antibiotic.
Generation of minicell producing strain from Shigella flexneri.
Glycerol is highly viscous and difficult to remove completely from the minicell suspensions.
A plasmid within a parent bacterial cell or minicell of the invention also may contain a reporter element.
The formulations also optionally contain a bispecific ligand for targeting the minicell to a target cell.
This inducible phenotype approach to minicell production has several distinct advantages over the mutant systems.
This resulted in a clear diffuse minicell band Fig.
Generation of minicell producing strains from two different strains of Salmonella typhimurium.
The invention also provides bispecific ligands useful for targeting minicell vehicles to mammalian host cells.
The crude minicell preparation separated by the first density gradient centrifugation Fig.
This Example demonstrates that inducing bacteria to filament prior to filtration improves minicell purification processes.
The minicell band was collected and processed through a second Optiprep gradient Fig.
The individual drugs Doxorubicin and Paclitaxel also were visualized within the minicell cytoplasm.
The minicell bands Fig.
In one embodiment, the linking moiety is non-covalently attached to the minicell.
In one embodiment, the minicell comprises a detectable compound.
The minicell of claim 1, wherein said antibody or antibody derivative is an antibody.
In one embodiment, the minicell displays a binding moiety.
In one embodiment, the invention provides a pharmaceutical composition comprising the minicell.
Preferably, the minicell of the formulation contains the drug.
Thirty ex-conjugants were chosen and subcultured to test for maintenance of the minicell phenotype.
In another embodiment, the minicell is administered after the drug.
Also allude to the use of a third type of simplified cellular structure, a minicell protoplast.
To this end, conventional methods of minicell purification generally are unsatisfactory.
The minicell band was collected as before and re-processed through a third OptiPrep ™ gradient as above.
In another option, the minicell is administered after the drug.
In one embodiment, the method further comprises preparing a denuded minicell from the minicell.
In one embodiment, the minicell comprises a biologically active compound.
Thus, I-Ceul can be used in a broad range of different minicell producing parent strains.
